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统计优化硝化菌发酵培养基
引用本文:任杰,林炜铁,罗小春,谢明权.统计优化硝化菌发酵培养基[J].南方水产,2008,4(3):7-14.
作者姓名:任杰  林炜铁  罗小春  谢明权
作者单位:华南理工大学生物科学与工程学院,广东,广州,510006
摘    要:为提高硝化菌的亚硝酸盐氧化能力,利用统计试验设计(Plackett-Burman和Box-Behnken设计)优化得到一最佳培养基:NaHCO3 2.0g·L^-1;NaNO2 2.36g·L^-1;Na2C030.37g·L^-1;NaCl 0.34g·L^-1;KH2P040.05g·L^-1;MgSO4·7H2O 0.05g·L^-1;FeSO4·7H2O 0.03g·L^-1。在此条件下,硝化菌的最大亚硝酸盐氧化速率达到905.0mgNO2-N·(gMLSS·d)^-1(mixed liquor suspended solids,MLSS,混合液悬浮固体)。将50L降解速率为850mgNO2-N·(gMLSS·d)^-1的硝化菌(浓度为1.99gVSS·L^-1)(volatile solid,VSS,挥发性固体)投加至0.6hm^2的养殖水体中,7d内试验水体中的亚硝酸盐浓度即降至安全浓度以下。

关 键 词:硝化细菌  硝化菌(NOB)  优化  Plackett-Burman设计  响应面法  Box-Beknken设计
文章编号:1673-2227-(2008)03-0007-08
修稿时间:2008年2月28日

Statistical optimization of fermentation media for nitrite oxidizing bacteria
REN Jie,LIN Weitie,LUO Xiaochun,XIE Mingquan.Statistical optimization of fermentation media for nitrite oxidizing bacteria[J].South China Fisheries Science,2008,4(3):7-14.
Authors:REN Jie  LIN Weitie  LUO Xiaochun  XIE Mingquan
Institution:of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China)
Abstract:The statistical experimental design (Plackett-Burman and Box-Behnken design) was applied to optimize the culture medium of nitrite oxidizing bacteria for improving the nitrite oxidizing rate. Estimated optimum medium composition of the nitrite oxidizing rate was as follows: NaHCO32.0 g·L-1 ; NaNO22.36 g·L-1 ; Na2CO30.37 g·L-1 ; NaCl 0.34 g·L-1; KH2PO40.05 g·L-1 ; MgSO4·7H2O0.05 g·L-1 ; and FeSO4-7H2O0.03 g·L-1. The nitrite oxidizing rate reached a maximum at 905.0 nag NO2-N.( g MLSS.d)-1 ( mixed liquor suspended solids, MLSS). In the field trial, 50 L of nitrite oxidizing bacteria concentrate ( 1.99 g VSS·L-1 ) ( volatile solid, VSS) with 850 mg NO2-N-( g MLSS.d)-1 were added to 0.6 hectares of the aquaculture water. Nitrite level in all treated ponds remained very low compared to the steady increase observed in all of the control ponds during 7 days.
Keywords:nitrifying bacteria  nitrite oxidizing bacteria (NOB)  optimization  Plackett-Burman design  response surface method  Box-Behnken design
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