用IGF—ⅠmRNA表达量评价鲮饲料配方效果的研究

试验以秘鲁鱼粉为饲料蛋白源，研究4种蛋白水平（21．5％、32．2％、42．5％和51．6％）的饲料对鲮（Cirrhinus molitorella）生长、消化酶活性及类胰岛素生长因子（IGF—Ⅰ）mRNA表达水平的影响。经过8周的养殖试验，不同饲料蛋白水平下，蛋白酶活性与特定生长率（SGR）成极显著的正相关性（P〈0．01）；鲮肝组织中IGF—ⅠmRNA的表达量与特定生长率成极显著正相关性（P〈0．01）；鲮肝组织IGF—ⅠmRNA的表达量与蛋白酶活性也成极显著正相关性（P〈0．01）。结果表明，用鲮肝组织IGF—ⅠmRNA的表达量可以快速鉴定饲料的质量。

Research on assessing effects of diets of mud carp (Cirrhinus molitorella)using IGF-ⅠmRNA expression level

Using Peru fishmeal as the only dietary protein source, the effects of dietary protein level （21.5%, 32. 2% , 42. 5% and 51.6% ） on the growth, digestive enzyme activity and insulin-like growth factors-Ⅰ as well as IGF-Ⅰ mRNA expression level of mud carp （Cirrhinu.s molitorella） were investigated by using 4 formulations. After an 8-week growth trim under different dietary protein levels, the protease activity showed a very significant correlation to specific growth rate （P 〈 0. 01 ） ; the quantity of GH and IGF-Ⅰ mRNA in liver of mud carp showed a very significant correlation to specific growth rate （ P 〈0. 01 ） ; the quantity of IGF- Ⅰ mRNA expression in liver of mud carp showed a very significant correlation to the protease activity （ P 〈0.01）. The results indicated that IGF- Ⅰ mRNA expression level in liver was a suitable index for assessing the potential of new diets quickly.