饥饿对大口黑鲈消化器官、蛋白酶和淀粉酶活力的影响

对饥饿和饥饿后投喂的大口黑鲈(Micropterus salmoides)蛋白酶和淀粉酶活力及消化组织的变化进行了研究,饥饿周期为30d。饥饿对大口黑鲈消化道指数的影响为肝体比从试验的第3天开始明显下降(P<0.05),第20天开始保持稳定水平(P>0.05);幽门盲囊重与体重比先上升后下降;胃重与体重比始终呈上升趋势(P<0.05);肠重与体重比在第10天开始保持稳定水平(P>0.05);肠长与体长比呈上升趋势(P<0.05)。饥饿对蛋白酶活力影响为从开始到第3天胃蛋白酶活力上升,第10天开始下降;幽门盲囊蛋白酶活力在第10天之后持续下降(P<0.05);肠道蛋白酶活力一直呈下降趋势。饥饿对淀粉酶活力影响为肝脏淀粉酶活力、胃淀粉酶活力和幽门盲囊淀粉酶活力均呈下降趋势(P<0.05);肠道淀粉酶活力第10天之后即稳定在低水平(P>0.05)。饥饿后摄食对蛋白酶活力和淀粉酶活力的影响为饥饿后摄食蛋白酶活力和淀粉酶活力均呈上升趋势,上升的速度在不同组织有差异,在恢复摄食后的第10天基本达到正常水平。

Effects of starvation on digestive organs and activities of protease and amylase of largemouth bass (Micropterus salmoides)

An experiment was conducted to study the effects of starvation and refeeding on digestive organs and the activities of protease and amylase in largemouth bass (Micropterus salmoides). The course of starvation lasted for 30 days. From day 3 the liver weight/body weight (LW/BW) began decrease (P<0.05), and maintained a constant after 20 days of starvation (P>0.05). The pyloric caeca weight/body weight (PW/BW) grew up first, and then went down. The stomach weight/body weight (SW/BW) kept growing through the starvation period (P<0.05). The intestine weight/body weight (IW/BW) maintained stably for the first 10 days (P>0.05).The intestine length/body length (IL/BL) kept growing (P<0.05). The activity of protease in stomach was increasing for the first 3 days, and decreasing after day 10. The activity of protease in pyloric caeca was decreasing from day 10. The activity of protease in intestine decreased during the entire starvation period .The activities of amylase in liver,stomach and pyloric caeca all went down after starvation (P<0.05). The activity of amylase in intestine kept at lower level after day 10. When refeeding was applied, the activities of protease and amylase increased, and returned to normal level after 10 days of refeeding.