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重组鲮GH对鲮IGF-Ⅰ表达的影响
引用本文:张殿昌,黄燕琴,苏天凤,李建柱,朱彩艳,江世贵.重组鲮GH对鲮IGF-Ⅰ表达的影响[J].南方水产,2008,4(2):50-55.
作者姓名:张殿昌  黄燕琴  苏天凤  李建柱  朱彩艳  江世贵
作者单位:中国水产科学研究院南海水产研究所,广东广州,510300
基金项目:广东省自然科学基金 , 科技部科技基础条件平台建设计划
摘    要:使用半定量RT-PCR方法,研究了鲮(Cirrhinus molitorella)各组织中IGF-ⅠmRNA组织表达,并分析重组鲮GH处理后鲮IGF-Ⅰ表达变化情况。结果表明,鲮IGF-ⅠmRNA在肝组织中表达最高,其次是肾和脑,另外在肠、鳃、脾、性腺和心脏组织中也有表达,而在肌肉、皮肤中没有检测到鲮IGF-ⅠmRNA的表达;按120μg·g^-1鱼体重的剂量经腹腔注射重组鲮GH,观察12h后鲮IGF-Ⅰ表达变化情况,发现经重组鲮GH处理后,鲮肝组织IGF-ⅠmRNA表达水平显著升高,脑组织IGF-ⅠmRNA表达水平也稍有升高,而在肌肉中仍未检测到IGF-ⅠmRNA的表达;重组鲮GH处理前后,对照组中鲮血清中IGF-Ⅰ的含量为145.59±21.84ng·mL^-1,试验组中鲮血清中IGF-Ⅰ的含量为247.71±2.83ng·mL^-1,经t检验,P=0.043〈0.05,表明重组鲮GH处理前后鲮血清中IGF-Ⅰ的含量存在显著性差异。

关 键 词:  胰岛素样生长因子-Ⅰ  生长激素
文章编号:1673-2227-(2008)02-0050-06
修稿时间:2007年11月7日

The effect of recombinant growth hormone (GH) on insulin-like growth factor-I(IGF-I) expression in mud carp,Cirrhinus molitorella
ZHANG Dianchang,HUANG Yanqin,SU Tianfeng,LI Jianzhu,ZHU Caiyan,JIANG Shigui.The effect of recombinant growth hormone (GH) on insulin-like growth factor-I(IGF-I) expression in mud carp,Cirrhinus molitorella[J].South China Fisheries Science,2008,4(2):50-55.
Authors:ZHANG Dianchang  HUANG Yanqin  SU Tianfeng  LI Jianzhu  ZHU Caiyan  JIANG Shigui
Institution:(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)
Abstract:Expression patterns of IGF-ⅠmRNA in different tissues of mud carp(Cirrhinus molitorella)were studied using semi-quantitative RT-PCR method and the effects of recombinant mud carp growth hormone(rmcGH)on expression of IGF-Ⅰin mud carp were investigated.The IGF-ⅠmRNA was detected successfully in liver,kidney,brain,intestine,gill,spleen,gonad,and heart,and no IGF-ⅠmRNA was detected in skin and muscle.The IGF-Ⅰ mRNA detected was higher in liver than in other tissues.Mud carp were received either intra-peritoneal injections of rmcGH(120 μg·g^-1 body weight)or vehicle,tissue samples and blood were collected 12 hour later.Total RNA was isolated and assayed for IGF-ⅠmRNA using semi-quantitative RT-PCR.Blood was extracted to determine the levels of IGF-Ⅰ in serum.IGF-Ⅰlevels increased from 145.59±21.84 to 247.71±2.83 ng·mL^-1(P=0.043)in the serum after rmcGH injection.
Keywords:mud carp  Cirrhinus molitorella  insulin-like growth factor-Ⅰ  growth hormone
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