广东1个鲮原种群体的种质特征及遗传多样性分析

收集西江流域肇庆段的野生鲮鱼苗进行跟踪观察,记录其生长情况,测量形态参数,结果表明,该批鲮原种存在体色及生长性能等方面的分化一种体色淡黄,命名为子群体h,一种体色青,命名为子群体q;子群体h的生长性能优于子群体q。从2个子群体中各取24个个体进行RAPD分析。20条随机引物扩增共获得了179条清晰稳定的条带,其中多态性条带为82条。用popgen软件进行RAPD数据处理,结果是总群体的平均杂和度为0.1281,遗传距离为0.1232;子群体h的平均杂和度为0.1248,遗传距离为0.1151;子群体q的平均杂和度为0.1164,遗传距离为0.1010;2个子群体间的遗传距离值为0.1383。这个结果表明,该原种群体遗传多样性较高。

Germplasm characteristics and genetic diversity analysis of an original species population of mud carp (Cirrhinus molitorella) from Guangdong Province

Collecting wild mud carp population from the section of Zhaoqing, Xijiang River, Guangdong Province, succeeding observation, and recording morphological parameters, the results showed that two different stocks appeared in the river. Of the two, one with body color of light yellow were named after h, the other with body color of cyan named after q .The growth performance of stock h was better than stock q. Random amplified polymorphic DNA( RAPD) technique was applied to assess the genetic variations of the two stocks, 24 individuals respectively. Amplifications with 20 random primers gave 179 reproducible and stable fragments and 82 fragments of which was polymorphic. Based on RAPD data, the mean heterozygosity and genetic distance of the whole population was 0.1281 and 0.1232, the mean heterozygosity and genetic distance of stock h were 0.1248 and 0.1151, the mean heterozygosity and genetic distance of stock q were 0.1164 and 0.1010. The genetic distance between the two stocks was 0.1383. The results of RAPD analysis indicated that the genetic diversity of the original breed was relatively high.