斑节对虾细胞周期蛋白B慢病毒载体的构建及对Hela细胞增殖影响

细胞周期蛋白B（Cyclin B）是影响卵母细胞发育成熟的重要基因。文章构建了PmCyB慢病毒表达载体,并和慢病毒包装复合物在293T细胞中包装成慢病毒颗粒。利用包装好的慢病毒颗粒,感染干扰了Cyclin B1基因的Hela细胞,研究了PmCyB对Hela细胞增殖的影响。结果表明,通过siRNA干扰细胞自身的Cyclin B1后,转染对照组病毒液的Hela细胞增殖减缓;同时,干扰后转入含有PmCyB重组慢病毒表达载体,Hela细胞增殖速度明显要高于转染对照组病毒液的Hela细胞的增殖速度。这一结果表明,PmCyB基因的过表达能补偿Cyclin B1缺失导致的细胞增殖减缓的现象,说明PmCyB基因具有细胞周期调控功能,是细胞增殖与分化的重要调控基因,且功能可能与人类的Cyclin B1相似。人类的Cyclin B1是卵母细胞发育成熟的重要基因。由此可以推断,PmCyB基因可能对斑节对虾（Penaeus monodon）卵巢发育成熟起着重要作用。

Construction of lentiviral vector for overexpression of Penaeus monodon Cyclin B and its influence on Hela cells proliferation

Cyclin B plays an important role in oocyte development. The recombinant lentiviral vector of Penaeus monodon Cyclin B （PmCyB）was constructed. The recombinant lentiviral vector plasmid and Packaging Plasmid Mix were co-transfected into 293T cells to obtain recombinant lentivirus. After Cyclin B1 of Hela cells was interfered with siRNA,the recombinant lentivirus was transfected into these cells and its influence on the Hela cells proliferation was researched. The results show that Hela cells proliferation slowed down after siRNA interference,but renewed after being transfected by the packaged lentivirus particles containing PmCyB,which indicates that PmCyB gene has cell cycle activity and is important for regulation of cell proliferation and differentiation gene. The results also suggest that PmCyB might play an important role in ovarian development,as same as Homo sapiense Cyclin B1 which is an ovarian de-velopment essential gene.