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萘暴露对环文蛤的氧化胁迫与损伤研究
引用本文:窦勇,乔秀亭,陈丽梅,周文礼.萘暴露对环文蛤的氧化胁迫与损伤研究[J].南方水产,2014(4):39-44.
作者姓名:窦勇  乔秀亭  陈丽梅  周文礼
作者单位:天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384
基金项目:天津市科技兴海项目(KJXH2011-05);天津农学院科学研究发展计划项目(2013NO8)
摘    要:试验条件下研究了不同质量浓度(2 mg·L^-1、8 mg·L^-1和32 mg·L^-1)双环芳烃——萘暴露对环文蛤(Cyclina sinensis)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、碱性磷酸酶(AKP)活性和丙二醛(MDA)生成量变化的影响。结果表明,高浓度萘(32 mg·L^-1)在15 d内对环文蛤有很强的致死效应;在非致死条件下,随暴露时间延长SOD活性持续升高,CAT活力存在低浓度促进高浓度抑制的现象,AKP活性随暴露时间延长表现出先升高后降低的趋势,而MDA生成量则随暴露时间延长呈现低→高→低的变化。Pearson相关分析显示,在环文蛤应对氧化胁迫的过程中SOD和CAT呈现协同作用(R=0.439,P〈0.01),SOD和AKP表现拮抗关系(R=-0.571,P〈0.01)。在保护细胞膜结构完整性方面,CAT和AKP可能较SOD发挥更大作用(R=-0.490,P〈0.01)。

关 键 词:多环芳烃    环文蛤  氧化胁迫  生化指标

Study on oxidative stress and damage of Cyclina sinensis exposed to naphthalene
DOU Yong,QIAO Xiuting,CHEN Limei,ZHOU Wenli.Study on oxidative stress and damage of Cyclina sinensis exposed to naphthalene[J].South China Fisheries Science,2014(4):39-44.
Authors:DOU Yong  QIAO Xiuting  CHEN Limei  ZHOU Wenli
Institution:( Tianjin Key Lab. for Aquaculture Ecology and Cultivation, Fisheries College of Tianjin Agriculture University, Tianjin 300384, China)
Abstract:The SOD,CAT,AKP activities and MDA content were determined after Cyclina sinensis were exposed to different concen-trations(2 mg·L ^- 1 ,8 mg·L^ - 1 and 32 mg·L ^- 1 )of naphthalene under experimental ecotoxicology conditions. The results indicate that C. sinensis had high mortality rate under 32 mg·L^ - 1 of naphthalene. Under non-lethal conditions and with increase of naphthalene expo-sure time,SOD activity showed a continuous increasing trend;CAT activity was promoted in low-concentration group and inhibited in high-concentration group;AKP activity was initially increasing then decreasing;MDA content fluctuated following the trend of low→high→low. According to Pearson correlation analysis,SOD and CAT showed synergistic effect(R = 0. 439,P ﹤ 0. 01),while SOD and AKP showed antagonism effect(R = - 0. 571,P ﹤ 0. 01). CAT and AKP might protect cellular membrane structure better than SOD(R = - 0. 490,P ﹤ 0. 01).
Keywords:polycyclic aromatic hydrocarbon  naphthalene  Cyclina sinensis  oxidative stress  biochemical indicators
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