6种中草药对4种淡水鱼致病菌体外抑菌作用的研究

研究了6种中草药对4种淡水鱼致病菌温和气单胞菌（Aerom onas sobria）、嗜水气单胞菌（A.hydrophila）、生物Ⅰ-荧光假单胞菌（Pseudom onas fluorescentb iotypeⅠ）和柱状黄杆菌（Flavobacterium columnaris）的体外抑菌作用。以黄连（Coptis chinensis）、大黄（Rhem officinale）、连翘（Forsythia suspensa）、鱼腥草（Houttuynia corda-ta）、厚朴（Magnolia officinalis）和大青叶（Folium isatidis）6种中草药的水提物为研究对象,采用滤纸片法检测其对4种致病菌的抑制活性,并分别测定了这6种植物提取物对供试菌的最小抑菌浓度（M IC）。结果表明,黄连水提物对4种淡水鱼致病菌的抑菌效果最好,大黄和连翘水提物次之,而鱼腥草、厚朴和大青叶水提物的抑菌效果不明显。

In vitro antibacterial activity of 6 Chinese herbal medicines against 4 pathogens of freshwater fish

In this study,six Chinese herb medicines were tested for their antibacterial activity against 4 pathogens of freshwater fish： Aeromonas sobria,A.hydrophila,Pseudomonas fluorescent biotypeⅠand Flavobacterium columnaris.Using the aqueous extracts of 6 Chinese herbal medicines,Coptis chinensis,Rhem officinale,Forsythia suspensa,Houttuynia cordata,Magnolia officinalis and Folium isatidis,we measured the diameter of the zone of inhibition by a filter paper disc test and determined the minimal inhibitory concentration by concentration gradient method.Results indicate that the aqueous extract of C.chinensis has the strongest inhibitory effect on the 4 pathogens,then come R.officinale and F.suspensa,while H.cordata,M.officinalis and F.isatidis do not have significant inhibitory effects.