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| 湛江官渡近江牡蛎养殖水细菌群落的DGGE指纹分析 | |
| 引用本文： | 兰兰,刘广锋,王江勇,王瑞旋,刘文生.湛江官渡近江牡蛎养殖水细菌群落的DGGE指纹分析[J].南方水产,2012,8(5):31-38. |
| 作者姓名： | 兰兰 刘广锋 王江勇 王瑞旋 刘文生 |
| 作者单位： | 1. 华南农业大学,广东广州510642;中国水产科学研究院南海水产研究所,广东省渔业生态环境重点实验室,农业部南海渔业资源开发利用重点实验室,广东广州510300 2. 中国水产科学研究院南海水产研究所,广东省渔业生态环境重点实验室,农业部南海渔业资源开发利用重点实验室,广东广州510300 3. 华南农业大学,广东广州,510642 |
| 基金项目： | 现代农业产业技术体系建设专项资金-贝类产业技术体系科学家岗位专项经费，广东省农业攻关项目“贝类产业推进关键技术研究与示范” |
| 摘 要： | 2011年3月-12月利用变性梯充凝胶电泳（denatured gradient gel elctrophoresis, DGGE）技术对湛江官渡地区近江特蛎（Crassostrea ariakensis）养殖水细菌群落花流水组成进行了监测研究。UPGAMA聚类分析显示3月-12月的样品细菌主要分属2大类群；多样性指数结果显示3月-7月细菌多样性高于8月-12月；系统发育分析显示牡蛎养殖区水体优势菌群主要由以下种群组成：未可培养的变形菌门（Proteobacteria）（α、γ、δ、ε）、未可培养的拟杆菌门（Bacterioidetes）、未可培养的疣微菌门（Verrucomicrobiae bacterium）、未可培养的放线菌门（Actinobacteria）和未可培养的蓝细菌门（Cyanobacter bacterium）。最优势菌群为α-变形菌纲和拟杆菌门的黄杆菌属（Flevobacterium）。 |
| 关 键 词： | 近江牡蛎 养殖水 细菌群落 DGGE |
DGGE fingerprinting of bacterial community in aquaculture water of Crassostrea ariakensis from Guandu district, Zhanjiang city |
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| LAN Lan , LIU Guangfeng , WANG Jiangyong , WANG Ruixuan , LIU Wensheng.DGGE fingerprinting of bacterial community in aquaculture water of Crassostrea ariakensis from Guandu district, Zhanjiang city[J].South China Fisheries Science,2012,8(5):31-38. | |
| Authors: | LAN Lan LIU Guangfeng WANG Jiangyong WANG Ruixuan LIU Wensheng |
| Institution: | 1. South China Agricultural University, Guangzhou 510642, China; 2. Key Lab.of Fishery Ecology and Environment, Guangdong Province; Key Lab. of South China Sea Fishery Resources Exploitation ＆ Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China |
| Abstract: | We had monitored and investigated the bacterial community composition in aquaculture water for Crassostrea ariakensis in Guandu of Zhanjiang by denaturing gradient gel electrophoresis （DGGE） from March to December, 2011. UPCMA clustering analysis shows that there are 2 major populations of bacterial species. Diversity index reveals that the diversities in March~July were higher than those in August~December. Phylogenetic analysis indicates that the dominant microbial populations in the water are uncultured Proteobacteria （ α, γ, δ and ε）, uncultured Bacterioidetes, uncultured Verrucomicrobiae bacterium, uncultured Actinobacteria and uncultured Cyanobacter bacterium. Uncultured α-proteobacterium and Flevobacterium of uncultured Bacteroidetes are the most dominant bacteria. |
| Keywords: | Crassostrea ariakensis aquaculture water bacterial community DGGE |
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