| 加速溶剂萃取技术提取海刀豆中总黄酮的工艺研究 |
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| 引用本文： | 牛改改,邓建朝,李来好,杨贤庆,邓用川,吴燕燕,郝淑贤,戚勃.加速溶剂萃取技术提取海刀豆中总黄酮的工艺研究[J].南方水产,2014(1):78-85. |
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| 作者姓名： | 牛改改 邓建朝 李来好 杨贤庆 邓用川 吴燕燕 郝淑贤 戚勃 |
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| 作者单位： | [1]中国海洋大学食品科学与工程学院,山东青岛266000 [2]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心,广东广州510300 [3]海南大学农学院,海南海口570228 |
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| 基金项目： | 国家自然科学基金项目（21205138）;公益性行业（农业）科研专项经费项目（200903030）;海洋公益性行业科研专项（201005020-7） |
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| 摘 要： | 运用加速溶剂萃取技术（accelerated solvent extraction，ASE），通过单因素试验和响应面分析法优化海刀豆（Canavalia maritima）中总黄酮的提取工艺。在单因素试验的基础上，选取萃取温度、萃取时间和甲醇体积分数3个因素为自变量，总黄酮提取率为响应值，采用Box-Behnken试验设计方法，研究各因素的显著性及其交互作用对总黄酮提取率的影响。利用Design Expert软件，建立了提取率与各因素的二次多项式数学模型，得到了海刀豆中总黄酮的最佳提取条件：萃取压力10.3 MPa，萃取温度130 ℃，萃取时间16 min，甲醇体积分数66%，循环次数2次，总黄酮提取率为83.43%，与理论值相对偏差-1.63%。
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| 关 键 词： | 海刀豆 黄酮 加速溶剂萃取 响应面分析 |
Extraction of flavonoids from Canavalia maritima by accelerated solvent extraction |
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| NIU Gaigai,DENG Jianchao,LI Laihao,YANG Xianqing,DENG Yongchuan,WU Yanyan,HAO Shuxian,QI Bo.Extraction of flavonoids from Canavalia maritima by accelerated solvent extraction[J].South China Fisheries Science,2014(1):78-85. |
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| Authors: | NIU Gaigai DENG Jianchao LI Laihao YANG Xianqing DENG Yongchuan WU Yanyan HAO Shuxian QI Bo |
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| Institution: | 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China;2. Key Lab. of Aquatic Product Processing, Ministry of Agriculture; National R＆D Center for Aquatic Product Processing; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;3. College of Agriculture, Hainan University, Haikou 570228, China |
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| Abstract: | Based on a single-factor analysis, we used Response Surface Methodology （RSM） to optimize the method for extraction of flavonoids by accelerated solvent extraction（ASE）technology from Canavalia maritima. The extraction temperature,static extraction time and methanol concentration were chosen as independent variables and the flavonoids extraction rate was selected as response value; Box-Behnken method was adopted for the designment of the experiment to study the significance of each factor and its mutual influence on extraction rate. Then we utilized Design Expert software to establish the mathematical model of polynomial quadratic equation. The optimum conditions of flavonoids extraction were as follows： 130 ℃ of extraction temperature, 16 min of extraction time, 66% of methanol concentration and static circulate twice. The extraction rate was 83.43% at the optimum conditions and the relative error between the actual and predicted values was only 1.63%. |
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| Keywords: | Canavalia maritima flavonoids accelerated solvent extraction response surface methodology |
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