响应面法分析光照强度与盐度对卤虫休眠卵孵化率的联合效应

为了探明光照强度（L）与盐度（S）对卤虫（Artemia）休眠卵孵化率的联合影响，采用中心复合设计试验设计方案进行响应面分析，寻找最适的条件组合，并对预测的条件组合进行验证。结果表明，在该试验条件下，光照强度与盐度的一次效应、二次效应对孵化率均有极显著影响（ P ﹤0.01），两者的交互效应无显著影响（ P ﹥0.05），随着光照强度或盐度的上升，孵化率呈先升后降的变化趋势。孵化率对实际自变量 L 和 S 的二次多元回归方程的拟合程度高，可用于预测。光照强度2134.02 lx、盐度31.57 g·L -1为最适组合条件，在此最佳因子组合下卤虫休眠卵的孵化率达到最优，为29.86％，其可靠性达到0.983。对该组合条件进行结果验证，数据表明验证结果与预测值相符。建议该品系卤虫休眠卵在该研究得到最优的光照强度-盐度下进行孵化，以提高孵化效果。

Combined effect of light intensity and salinity on hatchability of Artemia cysts based on response surface methodology

Using central composite design and response surface methodology,we explored the combined effect of light intensity and salinity on hatchability of Artemia cysts as well as determined the optimum experimental combination. The results show that the linear and quadratic effects of light intensity and salinity had very significant effect on hatchability（P ﹤ 0. 01）under experimental conditions;no significant interaction was found between light intensity and salinity on hatchability（P ﹥ 0. 05）. The hatchability increased then decreased as light intensity or salinity increased. High fitting degree of hatchability to the quadratic regression equation of independent variables（light intensity and salinity）indicated its possibility of prediction. The optimum conditions for germination were light intensity of 2 134. 02 lx and salinity of 31. 57 g·L - 1 ,under which the hatchability reached 29. 86% and the reliability peaked to 0. 983. Moreover,since verification result of optimum conditions accorded with the predicted value,it is suggested that the hatchability of Artemia cysts can be improved under optimum light intensity and salinity.