养殖曼氏无针乌贼白色卵膜营养成分分析及评价

测定分析并评价了养殖曼氏无针乌贼（ Sepiella maindroni）白色卵膜（WEM）的营养成分。结果表明，WEM （鲜样）中水分、粗灰分、粗蛋白和粗脂肪的质量分数分别为92.34％、1.76％、5.28％和0.34％。WEM（干样）中氨基酸（AA）总量为55.53％，其中必需氨基酸（EAA）总量为19.85％；必需氨基酸与非必需氨基酸（NEAA）的比值为65.6％，构成比例符合 FAO/ WHO 规定的优质蛋白质标准；鲜味氨基酸（ DTAA）总量为29.53％。WEM的第一限制性氨基酸为苯丙氨酸＋酪氨酸（Phe ＋ Tyr），必需氨基酸指数（EAAI）为38.03。脂肪酸中多不饱和脂肪酸（PUFA）占19.21％。矿物质含量丰富，尤其以磷（P）质量分数最高（1423.2 mg·kg -1）。结果表明，WEM 营养组分较为全面，与野生黑色卵膜（BEM）的营养成分进行对比分析发现，在氨基酸组成、必需氨基酸和无机元素质量分数等方面存在差异，这种差异是否是导致白化受精卵孵化率降低的关键因素有待进一步研究。

Evaluation of nutritional composition in white egg membrane from cultured Sepiella maindroni

The nutritional composition of white egg membrane（WEM）from cultured Sepiella maindroni was determined in this paper. The results show that the contents of moisture,ash,crude protein and crude fat were 92. 34% ,1. 76% ,5. 28% and 0. 34% ,respectively. The total contents of amino acids（ AA）and essential amino acids（ EAA）in WEM（ dry weight）were 55. 53% and 19. 85% ,respectively. The ratio of essential amino acids to non-essential amino acids was 65. 6% ,which meets the high quality protein standard by FAO/ WHO. The total content of delicious amino acids（DTAA）was 29. 53% . The first limiting amino acid was Phe ＋ Tyr,and the essential amino acid index（EAAI）was 38. 3. There were 20 kinds of fatty acids in the crude fat with 19. 21% PUFA content. The contents of minerals and trace elements were rich,especially phosphorus（1 423. 2 mg·kg - 1 ）. The results reveal that WEM in cultured cuttlefish was potential marine food with low fat,full-bodied flavour and general nutrients. Remarkable changes were found in contents of amino acids,essential amino acids and mineral,compared with those of black egg membrane（BEM）from wild cuttlefish. Whether these changes were the key reasons causing decrease in white egg hatching rate needs further study.