首页 | 本学科首页   官方微博 | 高级检索  
     检索      

大黄鱼冰鲜杂鱼饲料中细菌多样性研究
引用本文:王勇忠,徐晓津,鄢庆枇,熊何健,邓霏,马英.大黄鱼冰鲜杂鱼饲料中细菌多样性研究[J].南方水产,2014(3):53-57.
作者姓名:王勇忠  徐晓津  鄢庆枇  熊何健  邓霏  马英
作者单位:[1]集美大学水产学院,农业部东海海水健康养殖重点实验室,福建厦门361021 [2]集美大学生物工程学院,福建厦门361021
基金项目:国家自然科学基金项目(31272669);厦门市科技局项目(2012S0486);厦门市海洋与渔业局项目(201309050001)
摘    要:为研究大黄鱼(Larimichthys crocea)冰鲜杂鱼饲料中细菌的多样性,用细菌通用引物构建了细菌16S rRNA基因克隆文库。从文库中随机挑选125个克隆子进行限制性片段长度多态性( RFLP)分析,得到82个不同的RFLP 带型。对部分代表性克隆子进行测序,得到23条有效序列。序列同源性分析和系统进化分析结果表明,大黄鱼冰鲜杂鱼饲料中细菌主要分为3大类群,其中γ-变形菌纲(γ-Proteobacteria)细菌占据明显地优势地位,约占所分析克隆子数的84.4%;其次是黄杆菌纲(Flavobacteria)细菌,约占10.9%;还有少量的梭杆菌纲(Fusobac-teria)细菌,约占4.7%。γ-变形菌纲细菌中以嗜冷杆菌(Psychrobacter sp.)最多,其次是发光杆菌(Photobacteri-um)。

关 键 词:大黄鱼  养殖饲料  细菌  16S  rRNA  基因  RFLP

Bacterial composition in feed mash of fresh frozen trash fish for large yellow croaker(Larimichthys crocea)
WANG Yongzhong,XU Xiaojin,YAN Qingpi,XIONG Hejian,DENG Fei,MA Ying.Bacterial composition in feed mash of fresh frozen trash fish for large yellow croaker(Larimichthys crocea)[J].South China Fisheries Science,2014(3):53-57.
Authors:WANG Yongzhong  XU Xiaojin  YAN Qingpi  XIONG Hejian  DENG Fei  MA Ying
Institution:1. Key Lab. of Healthy Mariculture for East China Sea, Ministry of Agriculture ; Fisheries College of Jimei University, Xiamen 361021, China; 2. Bioengineering College of Jimei University, Xiamen 361021, China)
Abstract:To examine the bacterial composition in the feed mash of large yellow croaker(Larimichthys crocea),we constructed a 16S rRNA gene clone library using universal primers for the domain bacteria. 125 clones randomly selected from the library were screened by restriction fragment length polymorphism(RFLP)analysis. These clones were divided into 82 different RFLP patterns. Some representative clones were sequenced and 23 sequences were obtained. Sequence homology and phylogenetic analysis reveal high bacterial diversity in large yellow croaker′s feed mash. The sequences were clustered into three major groups,in which γ-Proterbacteria were the most predominant group,accounting for about 84. 4% of the sampled clones;Flavobacteria were the second dominant group,account-ing for about 10. 9%. There were a small number of Fusobacteria,accounting for about 4. 7%. Psychrobacter sp. were the most abun-dant members within γ-Proterbacteria,and Photobacterium were the second abundant members.
Keywords:large yellow croaker  mariculture feed mash  bacteria  16S rRNA gene  RFLP
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号