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| 斑节对虾虾苗活力检测方法 | |
| 引用本文： | 杨其彬,李晓兰,孙苗苗,温为庚,黄建华.斑节对虾虾苗活力检测方法[J].南方水产,2010,6(1):12-18. |
| 作者姓名： | 杨其彬 李晓兰 孙苗苗 温为庚 黄建华 |
| 作者单位： | 1. 中国水产科学研究院南海水产研究所,广东,广州,510300 2. 华南农业大学,广东,广州,510642 3. 中国水产科学研究院南海水产研究所,广东,广州,510300;上海海洋大学,上海,201306 |
| 基金项目： | 农业行业标准项目(2009);;国家高技术研究发展计划(863计划)项目(2006AA603120);;国家支撑计划项目(2006BAD01A13-2,2007BAD29B01-9);;公益性行业(农业)专项(nyhyzx07-042);;广东省科技计划项目(2006A20204001) |
| 摘 要： | 该研究通过肉眼观察、镜检，进行干露、饥饿、盐度突降、福尔马林等抗性试验，并采用病毒检测等方法，以期建立评估斑节对虾（Penaeus monodon）虾苗活力和质量标准。结果表明，斑节对虾健康虾苗具有趋光性、集群性，体表光洁，肌肉透亮，肠胃食物充盈等特性。测试虾苗干露时间以15min为宜，健康虾苗干露后能立即恢复活力，而病弱虾苗多出现死亡、昏迷现象；虾苗的成活率随饥饿时间的延长而降低，随福尔马林浓度升高和时间延长而降低，随盐度突降幅度增加而降低。健康虾苗能忍受100～200μL·L^-1福尔马林溶液30min，成活率近100％；在盐度20～30下虾苗的成活情况较好，而其在淡水中仅能存活1h。对虾苗进行病毒检测，可以避免养殖中因虾苗携带病毒而可能导致的病毒性疾病的暴发。 |
| 关 键 词： | 斑节对虾 虾苗 活力 检测方法 |
Methods of testing vitality of postlarvae of Penaeus monodon |
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| YANG Qibin,LI Xiaolan,SUN Miaomiao,WEN Weigeng,HUANG Jianhua.Methods of testing vitality of postlarvae of Penaeus monodon[J].South China Fisheries Science,2010,6(1):12-18. | |
| Authors: | YANG Qibin LI Xiaolan SUN Miaomiao WEN Weigeng HUANG Jianhua |
| Institution: | 1. South China Sea Fisheries Research Institute/a>;Chinese Academy of Fishery Sciences/a>;Guangzhou 510300/a>;China/a>;2. South China Agricultural University/a>;Guangzhou 510642/a>;3. Shanghai Ocean University/a>;Shanghai 201306/a>;China |
| Abstract: | In the current study,to establish the standard system for assessing the activity and quality of larval Penaeus monodon,whose tolerance to drying,starving,sharply-decreased salinity and formalin were investigated by methodologies of visualizing,microscopy and virus detection.The results showed that healthy postlarvae were featured with phototaxis and gregariousness;their body-surface was clean and the tissue was transparent;the stomach and intestine were full.The appropriate time for postlarvae to live witho... |
| Keywords: | Penaeus monodon postlarvae vitality testing method |
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