斑节对虾7个全同胞家系间亲缘关系的微卫星分析

为了斑节对虾（Penaeus monodon）后续育种工作的需要,研究了斑节对虾7个全同胞家系的亲缘关系。从95对微卫星引物中筛选出16对,16个微卫星位点共发现79个等位基因（A）。每个位点的A为2～10个,平均4.9375,有效等位基因（Ne）1.7575～5.3980,平均3.3878,平均杂合度观测值（Ho）0.6679,平均杂合度期望值（He）0.6698。平均多态信息含量（PIC）0.6110。7个家系总近交系数（FIT）0.0099,家系内近交系数（FIS）-0.3725,家系间基因分化系数（FST）0.2709。根据遗传距离采用UPGMA法对7个家系进行聚类,F2和F6家系之间的遗传距离最小聚为一类,与F7家系遗传距离最远。结果表明,家系间基因交流较低,遗传分化程度较高,且很少发生近交,F7家系可以优先考虑与其他家系进行杂交选育。

Microsatellite analysis of genetic relationship among 7 full-sib families of Penaeus monodon

To further study the breeding strategy for Penaeus monodon,we investigated the genetic relationship among 7 full-sib P.monodon families.From 95 pairs,16 pairs of microsatellite primers were selected,and among 16 microsatellite loci 79 alleles were discovered.Each locus has 2～10 alleles and 4.937 5 on average;effective allele number ranges from 1.757 5 to 5.398 0 and 3.387 8 on average;average observed and expected heterozigosity value is 0.667 9 and 0.669 8,respectively;average polymorphism information content（PIC）is 0.611 0.Global heterozygote deficit across 7 families（FIT） reaches 0.009 9,inbreeding coefficient（FIS） is-0.372 5 and genetic differentiation coefficient（FST） reaches 0.270 9.According to the genetic distance,we conducted a clustering analysis of the 7 families by UPGMA.The results indicate that F2 and F6 cluster into one group with the minimum genetic distance,while the genetic distance between F2 and F7 is the furthest.Thus,the genetic differentiation among 7 families is relatively higher,with less gene flow and less inbreeding.F7 should be given prior consideration for hybrid breeding.