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3个鲤群体的微卫星标记与生长性状相关性分析
引用本文:刘伟,苏胜彦,董在杰,张建桥,马良骁,李灵玲,曲疆奇,袁新华.3个鲤群体的微卫星标记与生长性状相关性分析[J].南方水产,2012,8(3):17-24.
作者姓名:刘伟  苏胜彦  董在杰  张建桥  马良骁  李灵玲  曲疆奇  袁新华
作者单位:1. 南京农业大学无锡渔业学院,江苏无锡,214081
2. 中国水产科学研究院淡水渔业研究中心,农业部淡水渔业与种质资源利用重点实验室,江苏无锡214081
3. 南京农业大学无锡渔业学院,江苏无锡214081;中国水产科学研究院淡水渔业研究中心,农业部淡水渔业与种质资源利用重点实验室,江苏无锡214081;南京农业大学动物科技学院,江苏南京210095
4. 南京农业大学无锡渔业学院,江苏无锡214081;中国水产科学研究院淡水渔业研究中心,农业部淡水渔业与种质资源利用重点实验室,江苏无锡214081
基金项目:现代农业产业技术体系国家大宗淡水鱼类产业技术体系,国家科技支撑计划项目,中央级公益性科研院所基本科研业务费专项,江苏省自然科学基金
摘    要:利用筛选出鲤(Cyprinus carpio)的15对多态性较高的微卫星引物,分析其与黄河鲤(C.carpio haematopterus Temminck et Schlegel)、建鲤(C.carpio var.jian)和黑龙江野鲤(C.carpio haematopterus)群体中90个个体的体质量、体长、体厚和体高性状的相关性.利用SAS的一般线性模制(GLM)对15个微卫星标记与3个鲤群体的生长性状进行显著性检验。结果表明,Mfw5与黄河鲤的体高相关(P〈0.05);Hlj013、Cca09和Mfw7均与建鲤的体质量、体长和体高相关(P〈0.05);Mfw2与建鲤的体质量和体厚相关(P〈0.05);Mfw29与建鲤的体质量相关(P〈0.05);Mfw6与黑龙江野鲤的体质量、体长、体厚和体高均相关(P〈0.05);Mfw4与黑龙江野鲤的体质量、体长和体高相关(P〈0.05);Mfw11与黑龙江野鲤的体厚和体高相关(P〈0.05)。对同一性状不同基因型间进行多重比较,找到3个群体中与生长性状相关的基因型。

关 键 词:  微卫星  生长性状  相关分析

Correlation analysis of microsatellite DNA markers with growth trait among 3 breeding populations of common carp
LIU Wei , SU Shengyan , DONG Zaijie , ZHANG Jianqiao , MA Liangxiao , LI Lingling , Q Jiangqi , YUAN Xinhua.Correlation analysis of microsatellite DNA markers with growth trait among 3 breeding populations of common carp[J].South China Fisheries Science,2012,8(3):17-24.
Authors:LIU Wei  SU Shengyan  DONG Zaijie  ZHANG Jianqiao  MA Liangxiao  LI Lingling  Q Jiangqi  YUAN Xinhua
Institution:LIU Wei , SU Shengyan , DONG Zaijie , ZHANG Jianqiao , MA Liangxiao , LI Lingling , Q(U) Jiangqi , YUAN Xinhua
Abstract:We screened out the molecular makers of 15 mierosalellite loci for common carp( Cyprinus carpio), and compared these markers with Huanghe carp ( C. carpio haematopterus Temminck et Scblegel ), Jian carp ( C. carpio var. fian) and Heilongjiang carp ( C. carpio haematopterus) populations in aspects of body weight, body length, body width and body deptil. The results based on GLM show that Mfw5 is significantly correlated with body depth for Huanghe carp (P 〈 0. 05). For Jian earp population, Hlj013, Cca09 and Mfw7 are significantly correlated with body weight, body length and body depth ( P 〈 0. 05 ) ; Mfw2 is significantly correlated with body weight and body width (P 〈 0. 05 ) ; while Mfw29 is significantly correlated with body weight (P 〈 0. 05 ). For Heilongjiang carppopulation, Mfw6 is significantly correlated with body weight, body length, body width and body depth ( P 〈 0. 05) ; Mfw4 is signific, antly correlated with body weight, body length and body depth (P 〈 0. 05) ; Mfwl I is significantly correlated with hody width and body depth ( P 〈 0. 05 ). Furthermore, the genes and genotypes related to growth traits in the 3 carp populations are found.
Keywords:common carp  microsatellite  growth trait  correlation analysis
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