阿维菌素在鲈鱼肌肉组织中的残留与消除规律

为了解实际养殖过程中使用阿维菌素药物后,鲈（Lateolabrax japonicus）对阿维菌素的蓄积及消除规律,以指导阿维菌素药物在实际生产中的应用。以毒性试验为基础,在确定96 h半致死质量浓度（LC50）后,以0.25倍LC50,即指导用药量,质量浓度为4 ng·mL-1,结合实际养殖用药情况,药浴72 h后换水,观察阿维菌素在鲈鱼肌肉组织中的残留与消除规律。文章建立高效液相色谱-串联质谱法测定鲈鱼中阿维菌素残留量的方法,并以此法测定鲈鱼肌肉组织中阿维菌素的质量浓度。结果表明,随着药浴时间的延长,鲈鱼肌肉组织中的阿维菌素质量分数逐步增加,在药浴结束时达到峰值8.767μg·kg-1,随后阿维菌素在体内的残留量随消除试验的进行逐渐下降,至第528小时降至检测限以下。

Accumulation and elimination of avermectin in perch （Lateolabrax japonicus） muscle

To investigate the accumulation and elimination rules of avermectin in perch（Lateolabrax japonicus） muscle during actual breeding process,we determined the median lethal concentration in 96 h as 4 ng·mL-1 based on a toxicity test,and exposed the perch to 4 ng·mL-1 of avermectin for 72 h,and then transfered the solution into seawater.Thus,we develop a method by high performance liquid chromatography-tandem mass spectrometry to determine avermectin in perch muscle.The results show that the avermectin concentration in perch muscle gradually increases with passing medicated bath time,and reaches the peak of 8.767·kg-1 at the end of the medicated bath.Later,the avermectin in perch muscle gradually decreases and reduces below the detection limit at 528th hour.