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硫酸铵盐析法分离罗非鱼肌红蛋白的研究
引用本文:何俊燕,李来好,郝淑贤,杨贤庆,马海霞,岑剑伟,石红,胡强初.硫酸铵盐析法分离罗非鱼肌红蛋白的研究[J].南方水产,2009,5(2):17-22.
作者姓名:何俊燕  李来好  郝淑贤  杨贤庆  马海霞  岑剑伟  石红  胡强初
作者单位:1. 广东海洋大学食品科技学院,广东,湛江,524025;中国水产科学研究院南海水产研究所,广东,广州,510300
2. 中国水产科学研究院南海水产研究所,广东,广州,510300
3. 华南农业大学生命科学学院,广东,广州,510642
基金项目:国家自然科学基金,农业部948引进项目,广东省自然科学基金 
摘    要:通过测定盐析液的色泽、肌红蛋白含量、纯度、回收率和吸收光谱等的变化情况,分析了硫酸铵分级盐析过程对罗非鱼肌红蛋白分离的影响.研究表明,硫酸铵饱和度为60%~100%可导致肌红蛋白沉淀析出,其中饱和度为50%时,肌红蛋白纯度为37.52%,回收率仅为39.89%;饱和度大于60%时,肌红蛋白纯度虽有所降低,但回收率逐渐提高,当饱和度为100%时,回收率最高为87.56%.在盐析过程中,部分氧合肌红蛋白氧化成为高铁肌红蛋白.

关 键 词:肌红蛋白  罗非鱼  硫酸铵  分离

Separation of myoglobin from tilapia by ammonium sulfate salting-out
HE Junyan,LI Laihao,HAO Shuxian,YANG Xianqing,MA Haixia,CEN Jianwei,Shi Hong,HU Qiangchu.Separation of myoglobin from tilapia by ammonium sulfate salting-out[J].South China Fisheries Science,2009,5(2):17-22.
Authors:HE Junyan  LI Laihao  HAO Shuxian  YANG Xianqing  MA Haixia  CEN Jianwei  Shi Hong  HU Qiangchu
Institution:1. College of Food Science and Technology;Guangdong Ocean University;Zhanjiang 524025;China;2. South China Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Guangzhou 510300;3. College of Life Sciences;South China Agricultural University;Guangzhou 510642;China
Abstract:Effect of ammonium sulfate on salting-out of myoglobin was studied by analysing color,Mb content,Mb purity,recovery rate and absorption spectra.The results showed that the myoglobin could be precipitated after treating with ammonium sulfate at saturation 60%~100%,and the purity and recovery rate were 37.52% and 39.89% respectively after treated with 50% ammonium sulfate.Mb purity was slightly decreased but the recovery rate was increased gradually when the saturation was greater than 60%.And recovery rate w...
Keywords:myoglobin  tilapia  ammonium sulfate  separation  
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