鲮原种群体的AFLP分析

运用AFLP技术,对广东鲮鱼原种场保存的鲮原种2个子群体(子群体h、q)各29个个体进行遗传多样性分析。25对AFLP引物组合中的6对引物扩增共产生173条条带,其中多态位点数为72,多态位点比例为41.5%。在6对引物产生的173条扩增条带中,有一条扩增条带在子群体h中的出现频率远远高于其在子群体q中的出现频率(72.4%>20.6%),编号为E2M4-1。遗传多样性分析结果表明,子群体h的遗传多样性指数高于子群体q(0.1367>0.0998)。该研究为筛选鲮原种群体2个子群体间的特异性分子标记做了初步尝试。

AFLP analysis on original population of mud carp Cirrhinus molitorella

Amplified fragment length polymorphism(AFLP)technique was applied to assess the genetic variations of the original pop-ulation collected from Original Species Field of Cirrhinus molitorella in Guangdong Province.The studied samples contained sub-stock h and sub-stock q,29 individuals respectively.Amplifications with 6 pairs of primers gave 173 reproducible and stable fragments,of which 72 were polymorphic.There was one band whose frenquency was much higher in sub-stock h than that was in sub-stock q(72.4%>20.6%).We named it as E2M4-1.Based on AFLP data,we found that the genetic diversity of sub-stock h was higher than sub-stock q(0.1367>0.0998).This study is a preliminary attempt for special molecular marker selecting of the two wild sub-stocks.