| 鱼类肠道微生物总DNA的提取 |
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| 引用本文： | 汪明,刘军,陈爱敬,赵胜军,黄峰,刘立鹤.鱼类肠道微生物总DNA的提取[J].水利渔业,2010(3). |
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| 作者姓名： | 汪明 刘军 陈爱敬 赵胜军 黄峰 刘立鹤 |
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| 作者单位： | 动物营养与饲料科学湖北省重点实验室;武汉工业学院; |
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| 基金项目： | 湖北省教育厅重点项目(D200618007) |
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| 摘 要： | 尝试提取鲫(Carassius auratus)肠道菌群总DNA,为研究鱼类肠道菌群结构提供依据。以鲫肠道内容物为样本,用PBS多次洗涤,离心样品,沉淀菌体。使用试剂盒法提取肠道菌群总DNA,电泳结果显示,样品DNA条带明亮,无降解现象,可用于后续分子生物学试验研究;通过设计的细菌通用引物,对其16S rDNA基因进行PCR扩增,得到较清晰的图谱,条带整齐;表明采用该方法提取鱼类肠道微生物群落的DNA较为简单、准确、可行。
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| 关 键 词： | 鲫 肠道细菌 DNA提取 |
Genomic Total DNA Extraction from Carassius auratus Intestinal Microorganism |
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| WANG Ming,LIU Jun,CHEN Ai-jin,ZHAO Sheng-jun,HUANG Feng,LIU Li-he.Genomic Total DNA Extraction from Carassius auratus Intestinal Microorganism[J].Reservoir Fisheries,2010(3). |
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| Authors: | WANG Ming LIU Jun CHEN Ai-jin ZHAO Sheng-jun HUANG Feng LIU Li-he |
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| Institution: | WANG Ming,LIU Jun,CHEN Ai-jin,ZHAO Sheng-jun,HUANG Feng,LIU Li-he (Hubei key Laboratory of Animal Nutrition , Feed Science,Wuhan Polytechnic University,Wuhan 430023,China) |
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| Abstract: | Total DNA of intestinal tract bacteria from Carassius auratus intestinal were extracted to investigate intestinal microflora. Taking the intestinal substance of fish as sample to precipitate bacteria through PBS cleaning and centrifuge. Using Bacterial DNA Kit to extraction intestinal tract bacteria total DNA,subsequently used as templates in PCR. The electrophoresis implied that the banding-patterns of different samples were bright and non-degeneration phenomenon. The 16S rDNA was amplified from these DNA ... |
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| Keywords: | Carassius auratus Enterobacteria DNA extraction |
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