Sperm of feral carp <Emphasis Type="Italic">Cyprinus carpio</Emphasis>: optimization of activation solution |
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Authors: | Kazem Darvish Bastami Mohammad Reza Imanpour Seyed Hossein Hoseinifar |
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Institution: | (1) Fisheries Faculty, Gorgan University of Agricultural Sciences and Natural Resources, 45165-386 Gorgan, Iran;(2) Department of Fisheries and Environmental Sciences, Faculty of Natural Resource, University of Tehran, 31585-4314 Karaj, Iran |
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Abstract: | The spermatozoa of oviparous fish, such as feral carp (Cyprinus carpio), are immotile in the presence of semen plasma or isotonic solutions, and to obtain good motility, they must be diluted with
suitable medium. The objective of this study was to identify the best activating solution for feral carp sperm. Sperm motilities
were compared in the new activating solution (a): (50 mM NaCl, 30 mM KCl, 30 mM Tris, pH = 8.5) and activating solution (b):
(50 mM NaCl, 40 mM KCl, 30 mM Tris, pH = 8.5) based on effect of pH with everyone of Na+ and K+ ions versus four other activating solutions Billard’s saline solution, Poupard’s saline solution, distilled water and hatchery
water that is routinely used for extending carp semen. Our results showed that maximum total motility period and percentage
of motile sperm were seen in selected saline solution (a). The present study describes an activating solution that prolongs
feral carp sperm motility. |
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