首页 | 本学科首页   官方微博 | 高级检索  
     检索      

苯甲酸雌二醇诱导新吉富罗非鱼雌性化的研究
引用本文:万松良,魏志宇,任炳琛,张艳红,[竹夹]金华,雷晓中,万珊.苯甲酸雌二醇诱导新吉富罗非鱼雌性化的研究[J].水产学杂志,2012,25(4):29-32.
作者姓名:万松良  魏志宇  任炳琛  张艳红  [竹夹]金华  雷晓中  万珊
作者单位:1. 中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验,江苏无锡214081 湖北省水产研究所,湖北武汉430070
2. 湖北省水产研究所,湖北武汉,430070
3. 河北中捷国家级罗非鱼良种场,河北沧州,061108
4. 湖北省兽药监察所,湖北武汉,430070
基金项目:农业部淡水鱼类遗传育种和养殖生物学重点实验室开放课题开放基金,湖北省水产研究所科技发展基金
摘    要:2009年7月12日至10月21日和2010年5月1日至11月6日,水温23~31℃范围下,在3口2.72m×1.55m×0.72m、水深0.62m的水泥育苗池和3口1.2m×1.0 m×1.0 m、网目20目/英寸的小网箱中,分别放养100尾体长0.7~1.9㎝的新吉富尼罗罗非鱼(Oreochromis niloticus)苗,投喂含不同剂量的苯甲酸雌二醇(EB)激素的饲料,以获得用于生产YY超雄鱼的XY转化雌鱼。结果显示,用100 mg.kg-1EB饲料投喂26d、36d、46d、59d和饲养102d后,实验鱼的雌性率分别为80.0%、93.4%、93.9%和94.0%,分别比对照鱼(54.8%)高25.2%、38.6%、39.1%和39.2%;用200 mg.kg-1EB饲料投喂24d和饲养188d后,实验鱼的雌性率为83.1%,比对照鱼(46.9%)高出36.2%。经Χ2检验,两次试验的雌性转化率差异均极显著(P<0.001)。实验鱼和对照鱼的成活率差异不显著,未见畸形现象。实验结束时两批实验鱼的体质量和全长均显著小于对照鱼(P<0.01和P<0.05),未转化的实验雄鱼与对照雄鱼的全长、体质量差异不显著(P>0.50)。综合分析认为,用EB诱导新吉富罗非鱼苗雌性化的适宜剂量为200 mg.kg-1饲料,投喂35d左右较为适宜。

关 键 词:苯甲酸雌二醇(EB)  新吉富尼罗罗非鱼  雌性化  成活率  生长

Feminization of "Neojifu" Tilapia Oreochromis niloticus by Administration of Estradiol Benzoate
WAN Song-liang,WEI Zhi-yu,REN Bing-chen,ZHANG Yan-hong,JIA Jin-hua,LEI Xiao-zhong,WAN Shan.Feminization of "Neojifu" Tilapia Oreochromis niloticus by Administration of Estradiol Benzoate[J].Chinese Journal of Fisheries,2012,25(4):29-32.
Authors:WAN Song-liang  WEI Zhi-yu  REN Bing-chen  ZHANG Yan-hong  JIA Jin-hua  LEI Xiao-zhong  WAN Shan
Institution:1. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 2. Hubei Fisheries Science Research Institute, Wuhan 430070, China; 3. Hebei Zhongjie National Tilapia Farm, Cangzhou 061108, China; 4. Hubei Institute of Veterinary Drug Control, Wuhan 430070, China)
Abstract:Neojifu tilapia ( Oreochromis niloticus ) fry with body length varying from 0.7 cm to 1.9cm were fed diets containing different levels ofestradiol benzoate (EB) hormone to induce feminization ofXY neofemales for YY super males twice a daily from July 12th to October 21 st of 2009 and from May 1 st to November 6th of 2010. The fish fed the diet containing EB at a dose of 100 mg·kg^-1 were found to have 80.0% of females in 26 d, 93.4% of females in 36 d, 93.9% of females in 46 d, and 94.0% of females in 59 d and reared for 102 d, 25.2%, 38.6%, 39.1%, and 39.2% higher than that in the control group (54.8%), respectively. There was 83.1% of females in the fish fed the diet containing 200 ·kg^-1 EB for 24 d and reared for 188 d, 36.2% higher compared with the control group (46.9%), and very significant differences by X2 test in the two experiments (P〈0.001). There were no significant differences in the survival rate between the test fish and the control ones, and no abnormal fish were observed. It was found that the final body weight and final total length were very sig- nificantly and significantly smaller in the fish in the two experimental groups than those in the control group(P〈0.01, P〈0.05). There was no significant difference in growth between the experimental sex non-reversal males and the control males (P〉0.50). Thus, it is optimization of feminization protocol that neojifu tilapia fry are fed a diet containing EB at a dose of 200 mg·kg^-1 for about 35 d.
Keywords:estradiol benzoate (EB)  "neojifu" tilapia Oreochromis niloticus  feminization  survival rate  growth
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号