氟苯尼考及氟苯尼考胺在鲤体内的残留

在水温(18±1)℃下,采用高效液相色谱串联质谱法研究了氟苯尼考及其主要代谢物氟苯尼考胺在体质量(100±10)g鲤(Cyprinus carpio)体内的代谢及残留规律,以制定休药期。实验鱼间隔24h按15 mg.kg-1体质量的剂量口灌给药,连续给药3次,在给药后0.5h、1 h、2h、4h、8h、12h、24h、2d、3d、5d、7d、10d、15d、20d检测肌肉、皮肤内氟苯尼考和氟苯尼考胺的含量。结果表明:氟苯尼考在鲤体内的代谢速度快,2~4 h出现峰值;肌肉中氟苯尼考和氟苯尼考胺的残留浓度高于皮肤,肌肉和皮肤中氟苯尼考的含量均高于氟苯尼考胺,皮肤中的代谢速度较快。按欧盟标准相应的休药期不少于2d,按日本标准则不少于10 d。

Residues of Florfenicol and Florfenicol Amine in Common Carp in vivo

Residues of florfenicol and its major metabolite florfenicol amine were detected in vivo in common carp(Cyprinus carpio) with body weight of(100±10) g orally gavage administrated 3 times in a 24 h interval at a dose of 15 mg·kg-1 body weight by high performance liquid chromatography tandem mass spectrometry to evaluate the withdrawal period of the drug at water temperature of(18±1)℃.The contents of florfenicol and florfenicol amine were monitored in the muscles and skins 0.5h,1h,2h,4h,8h,12h,24h,2d,3d,5d,7d,10d,15d,and 20d after the drug delivery.The results showed that the florfenicol in the common carp was metabolized in vivo rapidly,peak in 2~4 hours.There was more florfenicol and its metabolite florfenicol amine residues in the muscles than those in the skin,and the florfenicol contents in the muscles and skins higher than the florfenicol amine residues in the same tissues,showing rapid metabolism in the skin.The withdrawal period of the drug was found to be over 2 d according to EU standard and over 10d according to Japanese standard.