首页 | 本学科首页   官方微博 | 高级检索  
     检索      

基于EST序列的鲤鱼生长相关SNP发掘
引用本文:张晓峰,杨晶,孙效文.基于EST序列的鲤鱼生长相关SNP发掘[J].水产学杂志,2009,22(4):1-7.
作者姓名:张晓峰  杨晶  孙效文
作者单位:1. 中国水产科学研究院黑龙江水产研究所,农业部水产生物技术重点开发实验室,黑龙江,哈尔滨,150070
2. 中国水产科学研究院黑龙江水产研究所,农业部水产生物技术重点开发实验室,黑龙江,哈尔滨,150070;大连水产学院生命科学与技术学院,辽宁,大连,116023
基金项目:黑龙江水产研究所基本科研业务费专项,国家重点基础研究发展计划 
摘    要:以本研究室454测序获得的EST序列和公共数据库中下载的共255,768条鲤鱼EST序列为源序列,鉴定出与生长相关的EST序列5,375条;采用QualitySNP软件,在31个EST重叠群中检测到69个可信度高的与生长相关的SNP,SNP发生频率为0.29/100个碱基。其中包括转换型35个,颠换型30个和缺失型4个。非同义SNP为46个,同义SNP为23个,二者比值为2.0。对所鉴定的SNP进行注释表明,这些包含SNP的生长相关基因归为20类,数量最多的为血纤维蛋白溶酶原基因4个、其次是载脂蛋白基因和磷酸甘油醛脱氢酶基因分别为3个。本研究所开发的SNP将促进鲤鱼生长性状的分子基础研究,为鲤鱼分子育种服务。

关 键 词:鲤鱼  生长  表达序列标签(EST)  单核苷酸多态(SNP)

Exploitation of SNPs Related to Growth Traits of Carp(Cyprinus carpio L.) Based on EST Sequences
ZHANG Xiao-feng,YANG Jing,SUN Xiao-wen.Exploitation of SNPs Related to Growth Traits of Carp(Cyprinus carpio L.) Based on EST Sequences[J].Chinese Journal of Fisheries,2009,22(4):1-7.
Authors:ZHANG Xiao-feng  YANG Jing  SUN Xiao-wen
Institution:ZHANG Xiao-feng1,YANG Jing1,2,SUN Xiao-wen1 (1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.College of Aqu-life Science and Technology,Dalian Fisheries University,DaLian 116023,China)
Abstract:In this study,we collected 255,768 EST sequences of common carp(Cyprinus carpio)from 454 sequencing and GenBank database.We identified 5,375 ESTs that related to growth trait.A total of 69 putative and reliable SNPs were detected within 31 genes by comparative analysis of QualitySNP software.The observed frequency of SNPs was 0.29 SNP per 100 bp.Among these SNPs,35 of which are transition,30 are tranversion and leaving other 4 as indels.The overall ratio of non-synonymous to synonymous SNPs was 2.0.Annotati...
Keywords:common carp  growth  expressed sequence tags(EST)  single nucleotide polymorphism(SNP)  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号