大泷六线鱼6个野生群体遗传多样性的12S rRNA基因分析

本文利用PCR技术扩增了6个野生群体大泷六线鱼线粒体12S r RNA基因的部分序列。在获得的582bp碱基序列中,共检测到314个变异位点,占总位点数的53.95%。34尾个体共定义了16种单倍型,6个群体的单倍型多样性指数为0.40~1.00,核苷酸多样性指数为0.00071~0.10441。中性检验Fu's Fs值为1.19782(P0.01)。分子变异分析表明:Fst=0.88326(P0.01),群体间和群体内变异分别为88.33%和11.67%。不同个体间的遗传距离构建的NJ和MP系统树表明,东港和旅顺群体亲缘关系较近,构成一个分支,其他群体构成另一分支。

Genetic Diversity Analysis of Six Wild Populations of Fat Greenling Hexagrammos otakii by Mitochondrial DNA 12S rRNA Partial Sequence

Nucleotide sequences of mitochondrial DNA 12S rRNA partial sequence were amplified in six wild populations of fat greenling Hexagrammos otakii using PCR techniques. A total of 314 variable sites were detected among the aligned sequences of 582 bp with mutation rate of 53.95%. Sixteen haplotypes were identified from 34 individuals according to the determined sequences, and the six populations had haplotype diversity of 0.40 ~ 1.00, nucleotide diversity of 0.00071 ~ 0.10441 and Fu's Fs of 1.19782 in neutral-ity tests(P < 0.01). AMOVA analysis demonstrated that: there were Fst = 0.88326(P < 0.01), variance of 88.33% among six popula-tions and variance of 11.67% within the six populations. The NJ and MP molecular phylogenetic trees constructed by the distances a-mong different individuals revealed that the phylogenetic trees were all divided into two branches, one in Donggang and Lvshun popu-lations and the others in the other populations.