患诺卡氏菌病的大黄鱼几种主要组织的酶活力变化分析

以酶学分析方法,测定患诺卡氏菌病的大黄鱼几种主要组织的酶活力变化。结果表明,与对照组相比,患病大黄鱼淀粉酶活力在心、脾中无明显变化,在肾、肝和鳃中均有极显著下降(P<0.01);溶菌酶活力在心、肾、肝、鳃中极显著升高(P<0.01),而在脾脏中极显著下降(P<0.01);碱性磷酸酶活力在心、脾、肝、鳃中明显升高,在肾脏中极显著下降(P<0.01);酸性磷酸酶活力在心、脾中较高,肾、肝中偏低,鳃中无明显变化;谷草转氨酶和谷丙转氨酶活力在肾脏中均极显著升高,肝脏中均极显著降低(P<0.01),而在心、脾、鳃组织中均无明显变化(P>0.05);丙二醛在肾脏和肝脏中含量均有升高,在心、脾、鳃中无明显变化;超氧化物歧化酶活力在肝和鳃中极显著下降(P<0.01),在心、脾、肾中无明显变化;过氧化氢酶活力在心和肾中显著降低,在脾脏中极显著升高(P<0.01),而在肝脏和鳃中无明显变化。由此可见,大黄鱼对诺卡氏菌侵染有明显的应激反应,且病原菌对病鱼器官有不同程度的损伤。

The analysis of enzymatic activities in several major tissues of Pseudosciaena croceas infected with nocardiosis

In order to reveal the effect of nocardiosis on Pseudosciaena croceas physiological conditions,the process of physiological and biochemical pathology was explored in this article. With the method of enzymology analysis, the change of enzyme activity in several main lesion tissues of P. croceas infected with Nocardia was tested. The results indicated that, in contrast with the control group, the amylase activities had no significant change in heart and spleen, while decreased apparently in kidney, liver and gill(P<0.01).Lysozyme activities in heart, liver, kidney and gill increased significantly(P<0.01), whereas declined significantly in spleen (P<0.01). Alkaline phosphatase activities in heart, spleen, liver and gill increased significantly, while became lower in kidney (P<0.01). Acidic phosphatase activities were higher in heart, spleen, and lower in kidney and liver,while no significant change in gill was found. Both glutamic-oxalacetic transaminease and glutamic-pyruvic transaminase increased significantly in kidney and decreased apparently in liver(P<0.01), while none of them showed significant change in heart, spleen and gill(P>0.05). Malondialdehyde both in kidney and liver decreased and had no significant changes in heart, spleen and gill. Superoxide dismutase activities reduced significantly in liver and gill(P<0.01), while no significant changes in heart, spleen.The catalase activities reduced significantly in heart and kidney, and increased in spleen(P<0.01), while no significant changes in liver or gill.Therefore, P. croceas had obvious stress response to Nocardia infection and showed different levels of damage to the organs of sicken fishes.