鱼鳞胶原-壳聚糖止血海绵的制备工艺 |
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作者姓名: | 王茵 黄煜 许永安 吴成业 |
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作者单位: | 1. 福建省水产研究所,厦门,361012 2. 福建农林大学食品科学学院,福州,350002 |
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基金项目: | 福建省自然科学基金,福建省水产研究所科研发展基金资助项目 |
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摘 要: | 以罗非鱼Tilapia mossambica鱼鳞为原料,经低温酶解提取鱼鳞Ⅰ型胶原,将鱼鳞Ⅰ型胶原与壳聚糖进行混合交联,冻干后制成胶原-壳聚糖止血海绵。以止血海绵的密度、吸水倍数、保水率、透气率和止血时间为指标,通过单因素实验和正交试验优化获得最佳的制备工艺:胶原与壳聚糖配比7∶3、制备液总浓度1.4%、交联剂添加量0.015%。在该最佳工艺下制备的止血海绵密度为22.95mg/cm3、吸水倍数为45.34、保水率为55.83%、透气率为46.36%、止血时间为68.0s,呈蜂窝状多孔结构,具有优良的持水性和透气性。
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关 键 词: | 鱼鳞胶原 壳聚糖 止血海绵 交联 |
收稿时间: | 2011/11/26 0:00:00 |
修稿时间: | 2012/1/20 0:00:00 |
Research on preparation technique of fish scale collagen-chitosan hemostatic sponge |
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Authors: | WANG Yin HUANG Yu XU Yong-an WU Cheng-ye |
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Institution: | 1* (1Fisheries Research Institute of Fujian,Xiamen 361012) (2College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 360002) |
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Abstract: | Fish scale type I collagen was extracted through low temperature enzymolysis from Tilapia mossambica, and after being crosslinked with chitosan, hemostatic sponge was generated via freeze-drying. Through single factor comparative tests and orthogonal tests, the optimum preparation technique was obtained as follows: mixing of collagen with chitosan according to a given volume ratio 7∶3, maintaining total concentration of preparation mixture at 1.4%, and keeping dosage of cross-linking agent at 0.015%. Under this condition, the generated hemostatic sponge possessed excellent properties, such as density (22.95 mg/cm3), water absorption (45.34), water retention (55.83%), breathability (46.36%), and hemostatic time (68.0 s). A faveolate vesicular structure, which contributed to preferable properties, was clearly observed with SEM. |
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Keywords: | Fish scale collagen Chitosan Hemostatic sponge Crosslinking |
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