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| 波纹唇鱼染色体制备及核型的初步研究 | |
| 作者姓名： | 周丽青 杨爱国 吴彪 程鹏 于宏 王华 雷霁霖 |
| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,青岛,266071 2. 中国水产科学研究院黄海水产研究所,青岛,266071;大连水产学院,116023 3. 中国检验认证集团山东有限公司,青岛,266071 |
| 基金项目： | 国家“863”计划项目(2006AA10A414)资助 |
| 摘 要： | 出于对波纹唇鱼这一濒危物种资源调查和保护的目的,探讨了活体状况下波纹唇鱼染色体制备的方法,并对其染色体进行分析。剪取1龄波纹唇鱼的微量尾部鳍条及对应部位的愈合增生组织为材料,采用热滴片法制备染色体标本。核型分析结果表明,波纹唇鱼二倍体染色体数目2n=48,核型为2n=4M+10SM+32ST+2T,因所取鱼的条数有限,且均为雌性,所以尚不能确定是否有异型性染色体的存在。 |
| 关 键 词： | 波纹唇鱼 染色体 鳍条 愈合增生组织 核型 |
| 收稿时间： | 2009/4/16 0:00:00 |
| 修稿时间： | 2009/6/30 0:00:00 |
A preliminary study on chromosome preparation and karyotype |
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| Authors: | ZHOU Li-qing YANG Ai-guo WU Biao CHENG Peng YU Hong WANG Hua LEI Ji-lin |
| Institution: | 1 Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 Dalian Fisheries University;116023;3 China Certification & Inspection Group Shandong Limited Corporation;Qingdao 266071 |
| Abstract: | In order to protect the humphead wrasse Cheilinus undulatus(Ruppell,1835),an endangered species,the metaphase chromosome preparations of the live fish and its karyotype were studied in this paper.The metaphase chromosome was obtained from the fin and its healing tissue of one-year old fish by hot air drying methods.The results showed that there were 48 chromosomes in diploid and their karyotype formulae was 2n=4M +10SM + 32ST +2T.Heterotypic sex chromosome was not found since the number of the tested fish w... |
| Keywords: | Humphead wrasse Cheilinus undulatus Chromosome FinHealing hyperplastic tissue Karyotype |
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