| 漠斑牙鲆引进群体子一代遗传多样性的RAPD分析 |
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| 作者姓名： | 李鹏飞 刘萍 柳学周 |
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| 作者单位： | 1. 农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,青岛,266071;中国海洋大学,生命科学与技术学部,青岛,266003
2. 农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,青岛,266071 |
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| 基金项目： | 国家基础条件平台项目 |
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| 摘 要： | 运用随机扩增多态性DNA（RAPD）技术对引进群体漠斑牙鲆Paralichthys lethostigma 子一代48个个体的遗传多样性进行了分析，筛选的40个10bp的随机引物中，25个引物产生了稳定性好，可重复性强的谱带。共检测到154个位点，其中多态位点有122个，多态位点的比例为79．22％，有效等位基因数为1．5629±0．3631，Nei＇s基因多样性指数为0．3184±0．1843，Shannon信息指数是0．4651±0．2574。结果表明，漠斑牙鲆处于较高的遗传多样性水平。
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| 关 键 词： | 漠斑牙鲆 遗传多样性 随机扩增多态DNA |
| 收稿时间： | 2007/11/17 0:00:00 |
| 修稿时间： | 2008/4/25 0:00:00 |
RAPD analysis of genetic diversity in first generation of introduced Paralichthys lethostigma |
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| Authors: | LI Peng-fei LIU Ping LIU Xue-zhou |
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| Institution: | LI Peng-fei LIU Ping LIU Xue-zhou (1. Key Laboratory for Sustainable Utilization of Marine Fishery Resources,Ministry of Agniculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;2. College of Life Science and Technology, Ocean University of China,Qingdao 266003) |
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| Abstract: | RAPD technique was used to analyze the genetic diversity of forty-eight fish samples from the first generation of introduced Paralichthys lethostigma. Twenty-five primers screened from 40 10bp primers produced highly repetitive and steady bands. 154 DNA fragments were identified, of which 79.22% （122 fragments） were polymorphic. The mean effective number of alleles per locus was 1. 562 9±0. 363 1,Nei＇s gene diversity index was 0. 318 4± 0. 184 3, and Shannon＇s information index was 0. 465 1±0. 257 4. All the results indicated that P. lethostigma had high genetic variability. |
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| Keywords: | Paralichthys lethostigma Genetic diversity RAPD |
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