| 几种前处理方法所获刀鲚(Coilia nasus)耳石年轮的效果比较 |
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| 作者姓名： | 姜 涛 刘洪波 卢明杰 陈婷婷 杨 健 |
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| 作者单位： | 1. 南京农业大学无锡渔业学院 无锡214081;中国水产科学研究院淡水渔业研究中心院长江中下游渔业生态环境和资源养护重点实验室 无锡 214081;2. 中国水产科学研究院淡水渔业研究中心院长江中下游渔业生态环境和资源养护重点实验室 无锡 214081;3. 上海海洋大学水产与生命学院 上海 201306;4. 南京农业大学无锡渔业学院 无锡214081 |
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| 基金项目： | 国家自然科学基金项目(31372533)、江苏省科技项目(自然科学基金)(BK20131089)和江苏省普通高校研究生科研创新计划项目(CXLX12_0266)共同资助 |
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| 摘 要： | 本研究较为系统地比较了刀鲚(Coilia nasus)耳石的几种碾磨方法及耳石酸蚀前处理所获刀鲚耳石年轮的效果.结果显示,耳石横截面碾磨法更易获取较为清晰的年轮,而矢状面碾磨法的效果不及前者,但后者在用于耳石微化学的研究中却更有优势.经5％EDTA处理过的耳石,比用1％HC1处理过的耳石,更容易获得较好的年轮效果.本研究还发现,刀鲚耳石年轮形成过程中的一些形态变化,与刀鲚生活史中的生境变化有关联性.
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| 关 键 词： | 刀鲚 耳石 年轮 前处理 |
| 收稿时间： | 2015/3/18 0:00:00 |
| 修稿时间： | 2015/5/14 0:00:00 |
The Comparison of Different Pre-treatment Methods for Acquiring Otolith Annuli of Coilia nasus |
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| Authors: | JIANG Tao LIU Hongbo LU Mingjie CHEN Tingting and YANG Jian |
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| Institution: | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081; Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,College of Fisheries and Life Science,Shanghai Ocean University, Shanghai 201306,Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081; and Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081; Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 |
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| Abstract: | Estuarine tapertail anchovy Coilia nasus has been a highly popular and commercially valuable anadromous species in China the texture of which is delicate. Recently age estimation by otolith of this fish has become a hotspot in ecological research, which largely depends on efficient pre-treatment methods that generate clearer annual rings in the otolith of C. nasus. In this study we compared the effectiveness of several pre-treatment methods. The results showed that the transverse plane was clearer than the sagittal plane through the core; however, the latter was more suitable in some special analysis (especially microchemistry). Etching with 5% EDTA produced a clearer surface than 1% HCl. Analysis on the rings in the sagittal planes after etching suggested that the morphological change in the otoliths was correlated with transition in the habitat during the lifetime. In conclusion, the transverse plane is suitable for determining the age, and the sagittal plane is optimal in microchemical analysis. |
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| Keywords: | Coilia nasus Otoliths Annulus Pre-treatment |
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