| 性成熟雌性牙鲆(Paralichthys olivaceus)新型膜孕激素受体(mPRL)的定性定量表达分析 |
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| 作者姓名： | 史 宝 柳学周 徐 涛 李晓妮 徐永江 张金勇 |
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| 作者单位： | 1. 农业部海洋渔业可持续发展重点实验室 青岛市海水鱼类种子工程与生物技术重点实验室 中国水产科学研究院黄海水产研究所 青岛266071;青岛海洋科学与技术国家实验室 海洋渔业科学与食物产出过程功能实验室 青岛 266071;2. 山东省渔业技术推广站 济南250013 |
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| 基金项目： | 国家自然科学基金项目(31201982)、国家鲆鲽类产业技术体系(CARS-50)和山东省优秀中青年科学家科研奖励基金项目(BS2013SW042)共同资助 |
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| 摘 要： | 在牙鲆(Paralichthys olivaceus)繁殖周期,新型膜孕激素受体基因(mPR-Like,mPRL)在脑-垂体-性腺轴的表达变化规律与性腺发育成熟密切相关.为进一步研究牙鲆mPRL作用机制,本研究对其mRNA和蛋白的表达特征进行了分析.应用实时定量PCR方法分析mPRL mRNA在卵子形成过程中的时序表达,发现牙鲆mPRL mRNA相对表达量的最高值出现在性成熟阶段卵巢的Ⅴ时相卵母细胞.原位杂交分析mPRL mRNA在繁殖相关组织中的细胞学定位,发现mPRL mRNA主要分布在牙鲆性成熟阶段卵巢的卵母细胞膜上;在脑组织的神经元附近区域mPRL mRNA阳性信号也较强.制备牙鲆mPRL的多克隆抗体,采用Western blotting方法检测牙鲆mPRL蛋白在不同组织的表达特性,发现mPRL蛋白表达量在卵巢和脑组织中相对较高,在肝脏、头肾、肾脏中表达量相对较少.免疫组化结果显示,牙鲆mPRL蛋白在卵巢和脑组织的细胞学定位与mPRL mRNA定位一致.牙鲆mPRL在繁殖相关组织的表达特征说明其参与卵母细胞成熟的过程,并通过内分泌方式参与牙鲆的繁殖调控.
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| 关 键 词： | 牙鲆 新型膜孕激素受体 mRNA表达 蛋白表达 |
| 收稿时间： | 2015/12/13 0:00:00 |
| 修稿时间： | 2016/1/12 0:00:00 |
Expression Characterization of the Novel Membrane Progestin Receptor (mPR-Like) in Sexual Maturation of Female Japanese Flounder (Paralichthys olivaceus) |
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| Authors: | SHI Bao LIU Xuezhou XU Tao LI Xiaoni XU Yongjiang and ZHANG Jinyong |
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| Institution: | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071,Shandong Province Fisheries Technology Extension Station, Jinan 250013,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071 and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; |
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| Abstract: | |
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| Keywords: | Paralichthys olivaceus mPR-Like mRNA expression Protein expression |
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