七带石斑鱼繁殖群体“突眼”症病原菌的分离与鉴定 |
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作者姓名: | 陈超 程波 于宏 王印庚 杨志 史成银 曲江波 赵丛明 徐静荣 姜卓 |
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作者单位: | 1. 中国水产科学研究院黄海水产研究所,青岛,266071 2. 大连水产学院生命科学与技术学院,116023 3. 新疆农业大学动物科学学院,乌鲁木齐,830052 4. 烟台技术开发区天源水产有限公司,265600 5. 天津立达海水资源开发有限公司,300381 |
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基金项目: | 国家863计划项目(2006AA10A414-3);;农业部948项目(2008-Z8)共同资助 |
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摘 要: | 2007~2008年间,对暴发"突眼"症的七带石斑鱼繁育群体,从细菌学、寄生虫学和病毒学等方面进行了全面的筛查和实验研究。从具有明显"突眼"症的七带石斑鱼病灶组织中分离出1株致病力极强的优势菌CB1008,经人工感染试验证实为此次七带石斑鱼"突眼"症的致病菌。该菌革兰氏染色呈阴性,电镜下观察菌体呈短杆状,极生单鞭毛。通过API-32E鉴定系统和菌体常规形态特征、培养特性和生理生化反应指标测定,以及16S rDNA测序分析等综合鉴定,菌株CB1008为弧菌属哈维氏弧菌Vibrio harveyi。药敏试验结果表明,该菌株对氯霉素、萘啶酸、四环素、氟哌酸和氟罗沙星等5种药物较为敏感。
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关 键 词: | 七带石斑鱼 “突眼”症 病原菌 哈维氏弧菌 |
收稿时间: | 2009/3/3 0:00:00 |
修稿时间: | 2009/4/9 0:00:00 |
Isolation and identification of a pathogenic bacterium from Epinephelus septemfasciatus with exophthalmic disease |
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Authors: | CHEN Chao CHENG Bo YU Hong WANG Yin-geng YANG ZhiSHI Cheng-yin QU Jiang-bo ZHAO Cong-ming XU Jing-rong JIANG Zhuo |
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Institution: | 1 Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 College of Animal Science;Agricultural University of Xinjiang;Urumqi 830052;3 Yantai Tianyuan Fisheries Corporation;265600;4 Lida Seawater Resources Development Corporation;Tianjin 300381;5College of Life Science and Technology;Dalian Fisheries University;116023 |
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Abstract: | A pathogenic bacterial strain CB1008 was isolated from the eyes and internal organs of seven-band grouper Epinephelus septemfasciatus.It was proved to be the pathogen of the diseased seven-band grouper by artificial infection test.Morphological observations showed that it was a short-rod and Gram-negative bacterium with single polar flagella.According to API-32E system,through traditional physiological and biochemical experiments and 16S rDNA gene homology comparison of the bacterium,the results showed that... |
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Keywords: | Epinephelus septemfasciatus Exophthalmic disease Pathogenic bacteria Vibrio harveyi |
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