首页 | 本学科首页   官方微博 | 高级检索  
     检索      

不同光照周期对日本囊对虾生长、蜕皮和糖代谢的影响
作者姓名:王想  任宪云  绳秀珍  李翠苹  李健
作者单位:中国海洋大学 海水养殖教育部重点实验室 青岛 266003;中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 青岛 266071;中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 青岛 266071;青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071;中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 青岛 266071;上海海洋大学水产与生命学院 上海 201306
摘    要:本研究比较分析了不同光照周期(全黑暗D、光照:黑暗10L:14D、光照:黑暗14L:10D、全光照L)对日本囊对虾(Marsupenaeus japonicas)生长、蜕皮和糖代谢的影响。结果显示,随着光照时间的延长,日本囊对虾的存活率(SR)、特定生长率(SGR)和蜕皮率(MF)逐渐降低,到第49天时,L组日本囊对虾死亡率达100%。实验结束时,D组SR、SGR和MF显著高于10L:14D组和14L:10D组(P<0.05),分别为(60.000±2.722)%、(6.966±0.686)%/d和(4.667±0.363)%/d,但10L:14D组和14L:10D组间的SR、SGR和MF差异不显著。不同光照周期对日本囊对虾体内蜕皮抑制激素(MIH)和蜕皮激素(EH)浓度有显著影响。第30天时,D组MIH浓度显著低于10L:14D、14L:10D和L组(P<0.05),10L:14D和14L:10D组MIH浓度显著低于L组(P<0.05),10L:14D和14L:10D组间差异不显著;第60天时,D组MIH浓度显著低于10L:14D和14L:10D组(P<0.05),但2组间差异不显著;随着光照时间的延长,日本囊对虾体内EH浓度不断降低,第30天时,D组EH浓度显著高于10L:14D、14L:10D和L组(P<0.05),10L:14D、14L:10D和L组相比差异不显著;第60天时,D组EH浓度显著高于10L:14D和14L:10D组(P<0.05),但2组间差异不显著。不同光照周期显著影响日本囊对虾的糖代谢,不同处理组血清中的5-羟色胺(5-HT)、高血糖激素(CHH)和肝胰腺中的糖原磷酸化酶(GP)的变化趋势与上述MIH浓度变化趋势一致,而肝胰腺中糖原合酶(GS)的变化趋势与MIH浓度变化趋势相反。研究表明,日本囊对虾最适宜生长的环境为全黑暗状态。

关 键 词:日本囊对虾  光照周期  生长  蜕皮  糖代谢
收稿时间:2019/9/2 0:00:00
修稿时间:2019/9/24 0:00:00

Effects of Different Photoperiods on Growth, Molting and Glucose Metabolism of Marsupenaeus japonicas
Authors:WANG Xiang  REN Xianyun  SHENG Xiuzhen  LI Cuiping  LI Jian
Institution:Key Laboratory of Mariculture Minisrtry of Education, Ocean University of China, Qingdao 266003; Yellow Sea Fisheries Research Institue, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071;Yellow Sea Fisheries Research Institue, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071;Yellow Sea Fisheries Research Institue, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306
Abstract:This study compared the effects of different photoperiods (D, 10L:14D, 14L:10D, L) on the growth, molting and glucose metabolism of Marsupenaeus japonicus. The results showed that different photoperiods had significant effects on the growth, molting, and glucose metabolism of M. japonicas. With the extension of illumination time, the survival rate (SR), specific growth rate (SGR), and molting rate (MF) of these shrimps decreased gradually. By day 49, the mortality of the shrimps in the group L reached 100%. At the end of the experiment, SR, SGR, and MF were significantly higher in the group D (60.000±2.722)% than in the 10L:14D (6.966±0.686)%/d and 14L:10D (4.667±0.363)%/d groups (P<0.05). However, the differences in SR, SGR, and MF between the 10L:14D and 14L:10D groups were not significant. Different photoperiods significantly affected the concentration of molting inhibitory hormone (MIH) and ecdysone hormone (EH) in Japanese prawns. On day 30, the concentration of MIH was significantly lower in the group D than in the 10L:14D, 14L:10D, and L groups (P<0.05), and the concentrations of MIH were significantly lower in the 10L:14D and 14L:10D groups than in the group L (P<0.05); the differences in the 10L:14D and 14L:10D groups were not significant. On day 60, the concentration of MIH was significantly lower in the group D than in the 10L:14D and 14L:10D groups (P<0.05), but the differences between the two groups were not significant. The concentration of EH in the shrimps decreased continuously with the extension of the illumination time. On day 30, the concentration of EH was significantly higher in the group D than in the 10L:14D, 14L:10D, and L groups (P<0.05); however, there were no significant differences between these three groups. On day 60, the concentration of EH was significantly higher in the group D than in the 10L:14D and 14L:10D groups (P<0.05), but the differences between the two groups were not significant. Different photoperiods significantly affected the glucose metabolism of shrimps, and the changes in serotonin (5-HT), crustacean hyperglycemic hormone, and glycogen phosphorylase levels in the hepatopancreas of different treatment groups showed the same trend as the concentration of MIH mentioned above, whereas the changes in glutamine synthetase levels in the hepatopancreas exhibited an exactly opposite trend. Taken together, these findings indicate that a dark state is the most suitable environment for optimum growth of the Japanese prawn.
Keywords:Marsupenaeus japonicas  Photoperiod  Growth  Molting  Glucose metabolism
点击此处可从《》浏览原始摘要信息
点击此处可从《》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号