南极磷虾及其制品中总砷含量的分析方法研究

本研究比较了电感耦合等离子体质谱法(ICP-MS)、氢化物发生原子荧光光谱法(HG-AFS)以及银盐法测定南极磷虾(Euphausia superba)及其不同形式制品总砷含量的适用性，并采用确定的检测方法对南极磷虾冻虾、南极磷虾粉和市售不同品牌南极磷虾油中的总砷含量进行了系统测定。结果显示，采用ICP-MS法、HG-AFS法及银盐法检测南极磷虾及其制品中的总砷含量，3种方法的加标回收率分别达到100.26%~110.48%、82.68%~98.50%和89.64%~94.89%，RSD均小于5%。 3种国标方法对于南极磷虾及其相关制品的测定结果基本一致，但与银盐法相比，ICP-MS法、HG-AFS法操作复杂，仪器昂贵。因此，银盐法可作为快速测定南极磷虾及其制品中总砷含量的首选方法。采用银盐法测得南极磷虾冻虾、虾粉和虾油中总砷含量分别为0.44~0.45 mg/kg、1.04~1.91 mg/kg、0.52~5.50 mg/kg。加快建立南极磷虾及其制品中无机砷定量及砷形态分析技术对于科学认识南极磷虾砷的安全性至关重要。

Study on the Analytical Method of Total Arsenic Content in Antarctic Krill (Euphausia superba) and Its Related Products

The applicability of three national standard methods including inductively coupled plasma mass spectrometry (ICP-MS), hydride generation atomic fluorescence spectrometry (HG-AFS), and silver salt method used to determine total arsenic content in Antarctic krill (Euphausia superba) and its related products were evaluated in this study. The accuracy of the ICP-MS, HG-AF, and silver salt methods were 100.26%~110.48%, 82.68%~98.50%, and 89.64%~94.89%, respectively, and the RSD was less than 5% in all cases. The results of the three national standard methods were consistent. However, compared with the silver salt method, the ICP-MS and HG-AFS methods were complicated and expensive. Therefore, the silver salt method could be chosen as a quick method for determining the total arsenic content in Antarctic krill and its related products. The content of total arsenic in Antarctic krill, Antarctic krill powder, and Antarctic krill oil were 0.44~0.45 mg/kg, 1.04~1.91 mg/kg, and 0.52~5.50 mg/kg, respectively. To accelerate the establishment of methods to evaluate arsenic morphology and estimate inorganic arsenic quantitatively in Antarctic krill and its related products will be important in the future.