| 海洋芽孢杆菌N11-8产蛋白酶的发酵条件优化 |
| |
| 作者姓名： | 朱祥杰 王震 苑志欣 郝建华 郑兰红 |
| |
| 作者单位： | 农业农村部极地渔业开发重点实验室 农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071；上海海洋大学水产与生命学院 上海 201306,农业农村部极地渔业开发重点实验室 农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071；上海海洋大学水产与生命学院 上海 201306,农业农村部极地渔业开发重点实验室 农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071,农业农村部极地渔业开发重点实验室 农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071,农业农村部极地渔业开发重点实验室 农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071 |
| |
| 摘 要： | 本研究采用响应面法(RSM)对南极海洋芽孢杆菌(Bacillus sp.)N11-8液体发酵产蛋白酶PBN11-8的发酵条件进行快速优化。通过单因素实验初步确定南极海洋芽孢杆菌N11-8产蛋白酶的最佳碳源和氮源分别为可溶性淀粉和蛋白胨；并通过Plackett-Burman(PB)设计对影响其产酶相关因素进行评估，筛选出具有显著效应的3个因素：温度、氮源和碳源；以最陡爬坡实验逼近至上述因子最大响应区域，进而采用RSM法对其最佳水平范围进行研究，确定最优发酵条件为：可溶性淀粉3 g/L，蛋白胨13.1 g/L，酵母浸粉2.9 g/L，NaCl 5 g/L，KH2PO4 1 g/L，FeCl3·6H2O 2 mmol/L，初始pH值为7.0，温度为34.0℃，转速为200 r/min，装液量为50 ml/250 ml，接种量为4%，培养时间为60 h。最终优化后的酶活达到90.5 U/ml，比初始酶活提高了23.6%。
|
| 关 键 词： | 芽孢杆菌 蛋白酶 Plackett-Burman设计 响应面法 |
| 收稿时间： | 2017/10/9 0:00:00 |
| 修稿时间： | 2017/11/14 0:00:00 |
Optimization of Fermentation Conditions of Bacillus sp. N11-8 on the Production of Protease PBN11-8 |
| |
| Authors: | ZHU Xiangjie WANG Zhen YUAN Zhixin HAO Jianhua and ZHENG Lanhong |
| |
| Institution: | Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 and Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 |
| |
| Abstract: | Bacillus sp.; Protease; Plackett-Burman design; RSM |
| |
| Keywords: | Bacillus sp Protease Plackett-Burman design RSM |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|
