Abstract: | In this study we investigated the effects of starvation on the growth and immunity of sea cucumber Apostichopus japonicus. Sea cucumber seedlings with the initial weight of (20±0.15) g were exposed to starvation stress for different periods (0, 10, 20, 30, 40, 50, and 60 d). Non-specific immunity including activities of acidic phosphatase (ACP), lysozyme (LZM), superoxide dismutase (SOD), respiratory burst (RB), and phagocytic activity of coelomic fluid were examined to determine the effects of starvation on the immune responses. The body weight, the ratio of viscera to body wall (VBWR), and the survival rate were measured to determine the effects on the growth. The ACP and LZM activities decreased along with the starvation time: after a 60-day starvation the ACP and LZM activities decreased by 47.06% and 17.57% respectively. The activities of SOD, RB and phagocytosis first increased and then decreased during the 60-day starvation, and they reached their highest values on the 20th, 20th and 10th day respectively. On the 60th day the activities of SOD, RB, and phagocytosis were significantly lower and reached 27.87%, 38.08%, 53.43% of their initial values respectively. The body weights of the sea cucumbers constantly decreased along with the starvation time and reached 68.08% of the initial value on the 60th day. The VBWR increased gradually during the experiment and reached 0.56 on the 60th day which was significantly higher than the initial value 0.44 (P<0.05). The loss rate of the body weight increased and the survival rate decreased along with the starvation time. There was a negative quadric linear correlation between the loss rate of the body weight and the survival rate. These results indicated that sea cucumbers could consume the nutrients in the body wall to meet their needs of energy during starvation. Long-term starvation might cause suppression of immune activities thus affect the health and growth of sea cucumbers. |