| 金乌贼(Sepia esculenta)早期发育阶段相关酶活性的变化 |
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| 作者姓名： | 刘长琳 刘思玮 赵法箴 陈四清 刘春胜 燕敬平 |
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| 作者单位： | 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 |
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| 基金项目： | 国家科技支撑计划(2011BAD13B08)和中国水产科学研究院黄海水产研究所级基本科研业务费(20603022013021)共同资助 |
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| 摘 要： | 采用生化方法测定了金乌贼(Sepia esculenta)不同发育阶段的受精卵以及0-10日龄幼体的胰蛋白酶、胃蛋白酶、碱性磷酸酶、脂肪酶和谷丙转氨酶5种酶比活力的变化.结果显示,可溶性蛋白浓度总体呈先上升后下降再上升的变化趋势,其中,在囊胚和原肠期(Ⅱ)最高,在初孵幼体(Ⅶ)中最低,含量分别为81.50 mg/g和4.24 mg/g.胰蛋白酶、胃蛋白酶和谷丙转氨酶比活力的变化趋势相近,总体呈先上升后下降再上升的变化趋势,其中,在胚胎发育阶段均能检测出这3种酶的活性,说明主要自母体获得,但活力相对较低;在初孵幼体中,这3种酶活性均显著升高,比活力分别为0.51 U/g、1.68 U/mg和37.84 U/g;5日龄幼体(Ⅷ)中,3种酶比活力均显著下降至出膜前水平;10日龄幼体(Ⅸ)中,3种酶比活力均有小幅度上升.碱性磷酸酶和脂肪酶比活力的变化趋势相近,总体呈上升趋势,其中,在胚胎发育阶段仅检测到这2种酶的极低活性,幼体孵出后2种酶的比活力才显著上升,标志着器官发育的逐步完善和消化能力的逐步增强.
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| 关 键 词： | 金乌贼 胚胎发育 可溶性蛋白 消化酶 |
| 收稿时间： | 2015/9/15 0:00:00 |
| 修稿时间： | 2015/11/9 0:00:00 |
Changes in Activities of Related Enzymes in Early Developmental Stages of Sepia esculenta |
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| Authors: | LIU Changlin LIU Siwei ZHAO Fazhen CHEN Siqing LIU Chunsheng and YAN Jingping |
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| Institution: | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 |
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| Abstract: | |
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| Keywords: | Golden cuttlefish Sepia esculenta Embryonic development Soluble proteins Digestive enzyme |
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