| 南极磷虾粗酶液中蛋白酶的基础酶学性质研究及抑制剂的开发 |
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| 作者姓名： | 陈怡炫 陈 昕 王 芳 惠彦行 盛 军 徐海波 徐甲坤 |
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| 作者单位： | 中国海洋大学化学化工学院;农业农村部极地渔业开发重点实验室中国水产科学研究院黄海水产研究所;上海海洋大学食品学院;青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室 |
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| 摘 要： | 本研究探讨了反应温度、pH、金属离子(Zn~(2+)、Mg~(2+)、Cu~(2+)、Fe~(3+)、Al~(3+)和Ca~(2+))对南极磷虾粗酶液中蛋白酶活性的影响,研究了苯甲基磺酰氟(PMSF)、EDTA·2Na、碘乙酰胺(IAM)和甲苯磺酰–苯丙氨酸氯甲基酮(TPCK)对蛋白酶活性的抑制效果。结果显示,南极磷虾粗酶液中蛋白酶的最适反应温度为40℃;最适反应pH值为8.0;当金属离子浓度为0.5 mmol/L时,Zn~(2+)、Mg~(2+)、Cu~(2+)、Fe~(3+)和Al~(3+)金属离子对蛋白酶酶活的抑制率分别为55.02%、55.02%、35.39%、20.67%和41.12%,而Ca~(2+)离子对蛋白酶活有明显的促进作用;PMSF和EDTA·2Na对蛋白酶酶活具有较为显著的抑制作用,PMSF的浓度为8.0 mmol/L时,抑制率为60%;EDTA·2Na浓度为0.6 mmol/L时,抑制率为86.67%;IAM在低浓度时有一定的抑制作用;TPCK低浓度时抑制效果不明显,浓度升高时有一定的抑制作用。在5~30℃的温度范围内,随着温度升高,EDTA·2Na对蛋白酶酶活的抑制效果逐渐增加。本研究阐明了影响南极磷虾粗酶液蛋白酶酶活的因素,开发了针对南极磷虾粗酶液中蛋白酶活性的抑制剂,为南极磷虾在食品领域的开发利用提供了基础理论数据。
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| 关 键 词： | 南极磷虾 粗酶液 基础酶学性质 抑制剂 |
| 收稿时间： | 2017/10/24 0:00:00 |
| 修稿时间： | 2017/12/5 0:00:00 |
Study on the Basic Enzymatic Properties of Protease in Crude Enzyme of Antarctic Krill and the Development of Inhibitors |
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| Authors: | CHEN Yixuan CHEN Xin WANG Fang HUI Yanxing SHENG Jun XU Haibo and XU Jiakun |
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| Institution: | College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100;Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;,Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306,Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;,College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100;Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;,Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;,College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100;Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; and Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;Laboratory for Marine Drugs and Byproducts of Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071 |
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| Abstract: | |
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| Keywords: | Antarctic Krill Crude enzyme Basic enzymatic properties Inhibitors |
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