| 三疣梭子蟹Drosha和Exportin 5基因克隆及其在性腺发育过程中的表达分析 |
| |
| 作者姓名： | 张小辉 孟宪亮 高保全 刘 萍 王竹青 张 杰 蔡 影 |
| |
| 作者单位： | 上海海洋大学水产与生命学院;青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室;农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 |
| |
| 摘 要： | 本研究利用RACE技术克隆了三疣梭子蟹(Portunus trituberculatus)Drosha和Exportin 5基因的cDNA全长序列,Drosha基因长度为3443 bp,编码1038个氨基酸,预测蛋白质包含2个相邻的RNA酶Ⅲ结构域(RⅢDa和RⅢDb)和1个双链RNA结合结构域(dsRBD)。Exportin 5基因全长为5000 bp,编码1208个氨基酸,预测蛋白质包含1个Importin-βN-末端结构域(IBN-N)和1个核输出蛋白结构域(XPO-1)。同源分析显示,三疣梭子蟹Drosha氨基酸序列与其他物种高度相似,与日本对虾(Marsupenaeus japonicus)相似度最高,达94%。qRT-PCR分析结果显示,Drosha和Exportin 5基因在三疣梭子蟹各组织中均有表达,其在卵巢和肝胰腺中的表达量最高(P0.05);在精巢不同发育时期,2个基因表达量的变化趋势一致,均在Ⅱ期(精母细胞增殖、分化期)表达量最高;在卵巢发育过程中,2个基因表达量的变化趋势也大致相同,随着卵巢发育(Ⅱ~Ⅴ期)逐渐升高。研究表明,Drosha和Exportin 5基因可能通过调控miRNA的合成来影响三疣梭子蟹性腺发育过程,为深入解析三疣梭子蟹性腺发育调控机制提供了重要参考。
|
| 关 键 词： | 三疣梭子蟹 Drosha Exportin 5 基因克隆 基因表达 |
| 收稿时间： | 2017/4/1 0:00:00 |
| 修稿时间： | 2017/4/26 0:00:00 |
Molecular Cloning of Drosha and Exportin 5 and Their Expression During Gonadal Development in the Swimming Crab (Portunus trituberculatus) |
| |
| Authors: | ZHANG Xiaohui MENG Xianliang GAO Baoquan LIU Ping WANG Zhuqing ZHANG Jie and CAI Ying |
| |
| Institution: | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Laboratory for Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 |
| |
| Abstract: | |
| |
| Keywords: | Portunus trituberculatus Drosha Exportin 5 Gene cloning Expression analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|
