投喂频率对点带石斑鱼生长和血浆抗氧化指标的影响

为寻找工厂化养殖点带石斑鱼的适宜投喂频率,研究了不同投喂频率对点带石斑鱼初始体重(238.99±5.32)g]生长和血浆抗氧化指标的影响。投喂频率设1次/d(F_1)、2次/d(F_2)、3次/d(F_3),每次饱食投喂,连续喂食试验鱼28 d、56 d和84 d后采样。结果显示,随着投喂频率的增加,0~28 d、29~56 d和57~84 d三个阶段的增重率、特定生长率均显著增加(P0.05)。0~28 d和29~56 d阶段F_2组的蛋白质效率显著高于F_1和F_3组(P0.05),57~84 d时F_2组显著高于F_1组(P0.05),与F_3组差异不显著(P0.05);0~28 d阶段F_2组的饲料效率显著高于F_1和F_3组(P0.05);29~56 d和57~84 d阶段F_2组饲料效率显著高于F_1组(P0.05),与F_3组无显著差异(P0.05)。28 d时投喂频率对血浆抗氧化指标均无显著影响(P0.05);56 d时投喂频率对血浆总抗氧化能力(T-AOC)和过氧化氢酶(CAT)无显著影响(P0.05);F_1组总超氧化物歧化酶(T-SOD)显著高于F_3组(P0.05),与F_2组无显著差异(P0.05);F_3组的丙二醛(MDA)含量显著高于F_1组(P0.05),F_1组与F_2组无显著差异(P0.05);F_2组的谷胱甘肽过氧化物酶(GSH-PX)显著高于F_1组(P0.05),与F_3组差异不显著(P0.05)。84 d时投喂频率对血浆T-AOC和MDA无显著影响(P0.05);F_2组血浆T-SOD、CAT显著高于其它组(P0.05),血浆GSH-PX显著高于F_1组(P0.05),与F_3组差异不显著(P0.05)。研究表明,初始体重为(238.99±5.32)g的点带石斑鱼,适宜投喂频率为2次/d。

Effects of feeding frequency on the growth and plasma antioxidant indices of Epinephelus malabaricus

In order to find out the optimal feeding frequency for factory farming Epinephelus sp, this experiment investigated the effects of feeding frequency on the growth and plasma antioxidant indices of Epinephelus malabaricus initial body weight of (238.99 ±5.32) g].The fish were divided into 3 groups:F1 , F2 and F3 , which were respectively fed 1, 2 and 3 times every day and fed to satiety each time.Samples were successively collected and analyzed after feeding 28 d, 56 d, and 84 d.The results showed that during the three periods of 0-28 d, 29-56 d and 57-84 d, as feeding frequency increasing, the weight gain rate and specific growth rate increased significantly (P<0.05).During 0-28 d and 29-56 d, the protein efficiency ratio (PER) of F2 was significantly higher than that of F1 and F3(P<0.05), while during 57-84 d, the PER of F2 was significantly higher than that of F1 ( P<0.05) but had no significant difference from that of F3 ( P>0.05).Feed efficiency of F2 was significantly higher than that of F1 and F3 during 0-28 d (P<0.05), while during 29-56 d and 57-84 d, was higher than that of F1 ( P<0.05) but had no significant difference with that of F3 ( P >0.05).By the 28th d, plasma antioxidant indices were not significantly affected by feeding frequency (P>0.05).By the 56th d, T-AOC and CAT of plasma showed no significant difference among the groups (P>0.05), while T-SOD of F1 was significantly higher than that of F3 (P <0.05) but had no significant difference from F2(P>0.05), MDA of F3 was significantly higher than that of F1(P<0.05) but that of F1 and F2 had no significant difference ( P>0.05) , and GSH-PX of F2 was significantly higher than that of F1(P<0.05) but that of F3 and F1, F3 and F2 had no significant difference (P>0.05).By the 84th d, plasma T-AOC and MDA were not significantly affected by feeding frequency (P >0.05), while plasma T-SOD and CAT of F2 were significantly higher than those of F1 and F3(P<0.05), and plasma GSH-PX of F2 significantly higher that of F1 (P<0.05) and had no significant difference (P>0.05) from F3. Results of the present study suggest that the optimal feeding frequency is 2 times/d in the culture of Epinephelus malabaricus with an initial body weight of about 239 g.