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棒叶蕨藻变种对重金属Cu~(2+)、Pb~(2+)和Cd~(2+)胁迫的生理响应
引用本文:王荣霞,黄敏,陈傅晓,谭围.棒叶蕨藻变种对重金属Cu~(2+)、Pb~(2+)和Cd~(2+)胁迫的生理响应[J].渔业现代化,2017(2).
作者姓名:王荣霞  黄敏  陈傅晓  谭围
作者单位:1. 海南省海洋与渔业科学院,海南 海口,570203;2. 海南省海洋与渔业科学院,海南 海口570203;海南省热带海水养殖技术重点实验室,海南省热带海水养殖工程技术研究中心,海南 海口570203;3. 海南省海洋与渔业科学院,海南 海口570203;南海生物资源开发与利用协同创新中心,广东 广州510275
基金项目:海南省重点研发计划项目(ZDYF2017027),海南省自然科学基金项目(20164167)
摘    要:为研究棒叶蕨藻变种(Caulerpa sertularoides f.Longipes)对重金属离子胁迫的生理响应,通过恒温光照培养方法探讨棒叶蕨藻变种在重金属铜离子(Cu~(2+))、铅离子(Pb~(2+))、镉离子(Cd~(2+))的胁迫下对其日特定生长率(SGR)、可溶性蛋白含量、叶绿素a(Chl-a)、谷胱甘肽转移酶(GST)和超氧化物歧化酶(SOD)等指标的影响。结果显示,3种重金属离子的胁迫会影响藻体的正常生长,SGR均出现负增长,可溶性蛋白和Chl-a含量随处理浓度的增大而呈下降趋势,其中Cu~(2+)的影响尤为明显;重金属对藻体生长的抑制能力大小为Cu~(2+)Pb~(2+)Cd~(2+);3种重金属离子对SOD和GST影响均表现为先升后降趋势,除了Cd~(2+)对SOD影响峰值出现在5.00 mg/L之外,其他处理组的峰值均出现在质量浓度为2.50mg/L时。研究结果可为棒叶蕨藻变种的重金属胁迫机理研究提供参考。

关 键 词:棒叶蕨藻变种  重金属  胁迫  生理响应

The physical responses of Caulerpa sertularoides f. Longipes to heavy metal ( Cu2+、Pb2+、Cd2+) stress
Abstract:In order to study the physiological responses of the algae Caulerpa sertularoides f.Longipes to heavy metal (Cu2+,Pb2+,Cd2+) stress,constant-temperature illuminating cultivation method has been adopted to explore the influence of the stress from Cu2+,Pb2+ and Cd2+ on the specific growth rate (SGR),content of soluble protein, chlorophyll-a (Chl-a),glutathione-S-transferase (GST) and superoxide dismutase (SOD) of the algae.The results show that stress of the three kinds of heavy metal ions affects the normal growth of the algae;SGR showed negative growth;soluble protein and Chl-a contents decreased when the content of heavy metal ions increased;the influence of Cu2+was most obvious.The inhibition ability of heavy metals on the growth of algae was as follows:Cu2+>Pb2+>Cd2+.Both the SOD and GST contents,under the influence of the three heavy metal ions,increased first and then declined;the peak effect on the SOD concentration appeared when the concentration of Cd2+was 5 mg/L,but in other treatment groups the peak effect appeared at the concentration of 2. 50 mg/L. This study will provide a scientific basis for the study on the mechanism of heavy metal stress to Caulerpa sertularoides f.Longipes.
Keywords:Caulerpa sertularoides f  Longipes  Heavy metal  stress  physiological responses
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