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3种不同规模化培养模式对湛江等鞭金藻生产力及色素含量的影响
引用本文:吕布,杨守国,VASQUEZ Hebert Ely,王爱民,郑兴,顾志峰.3种不同规模化培养模式对湛江等鞭金藻生产力及色素含量的影响[J].渔业现代化,2022(1):11-17.
作者姓名:吕布  杨守国  VASQUEZ Hebert Ely  王爱民  郑兴  顾志峰
作者单位:海南大学海洋学院;海南大学南海海洋资源利用国家重点实验室;海南省海洋与渔业科学院
基金项目:国家自然科学基金项目(31772847);国家重点研发计划项目(2018YFD0900704);海南省自然科学青年基金(320QN207)。
摘    要:为进一步提高湛江等鞭金藻室外规模化培养效率,对管道式光生物反应器、聚乙烯桶和水泥池3种微藻室外规模化培养模式下湛江等鞭金藻的藻密度进行了评估比较.试验将接种藻密度控制为10.0×104 cells/mL,培养周期为16 d.结果显示:管道式光生物反应器培养模式下,生产力相对最高为0.0543 g/L/d、比生长速率为0...

关 键 词:湛江等鞭金藻  规模化生产  光生物反应器  水泥池  聚乙烯桶  生产力  光合色素

Effects of three different culture modes on productivity and pigment concentration of Isochrysis zhanjiangensis
LYU Bu,YANG Shouguo,VASQUEZ Hebert,Ely,WANG Aimin,ZHENG Xing,GU Zhifeng.Effects of three different culture modes on productivity and pigment concentration of Isochrysis zhanjiangensis[J].Fishery Modernization,2022(1):11-17.
Authors:LYU Bu  YANG Shouguo  VASQUEZ Hebert  Ely  WANG Aimin  ZHENG Xing  GU Zhifeng
Institution:(Ocean College,Hainan University,Haikou 570228,Hainan,China;State Key Laboratory of Marine Resource Utilization in South China Sea,Hainan University,Haikou 570228,Hainan,China;Hainan Academy of Ocean and Fisheries Sciences,Haikou 571126,Hainan,China)
Abstract:In order to improve the efficiency of outdoor large-scale culture of Isochrysis zhanjiangensis,comparation of cell density for I.zhanjiangensis large-scale outdoor cultivation was conducted in present study,under three modes that including pipeline photobioreactor,polyethylene bucket and cement tank.The density of inoculated algae was 10.0×104 cells/mL and the cultivation experiment was lasted for 16 d.And in pipeline photobioreactor mode for I.zhanjiangensis,the highest productivity and specific growth rate were 0.0543 g/L/d and 0.2767 d-1 respectively,with 3.70×106 cells/mL for cell density and 1.90 mg/L,0.94 mg/L,0.95 mg/L,for total chlorophyll contents,chlorophyll a and b respectively.The maximum biomass dry weight was observed to 0.7270 g/L,while 159.1255 mg/L for the maximum total protein content and 42.8627 mg/L for the maximum soluble sugar content.The best production was gain under the pipeline photobioreactor mode,followed by the cement pond mode.The pipeline photobioreactor mode is more helpful to improve productivity and accumulation for biomass and cell chlorophyll,compared with polyethylene bucket and cement tank culture mode.Furthermore,pipeline photobioreactor mode is easier to operate with high space utilization.In conclusion,pipeline photobioreactor mode is more suitable for I.zhanjiangensis large-scale outdoor cultivation,and support the development of mechanized and intelligent technology for microalgae production in the future.
Keywords:Isochrysis zhanjiangensis  large-scale production  photobioreactor  cement tank  polyethylene bucket  productivity  photosynthetic pigment
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