| 土壤中单宁和植酸降解菌的筛选、鉴定及液态发酵研究 |
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| 引用本文： | 王晓东,胡海滨,麦康森,张彦娇,徐玮,艾庆辉,张文兵.土壤中单宁和植酸降解菌的筛选、鉴定及液态发酵研究[J].水产学报,2016,40(10):1634-1642. |
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| 作者姓名： | 王晓东 胡海滨 麦康森 张彦娇 徐玮 艾庆辉 张文兵 |
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| 作者单位： | 中国海洋大学农业部水产动物营养与饲料重点实验室,教育部海水养殖重点实验室,山东青岛266003 |
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| 基金项目： | 教育部博士点专项基金（20120132120025）；山东省优秀中青年科学家科研奖励基金计划（BS2013SW007）；国家自然科学基金课题（31202006） |
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| 摘 要： | 为从土壤中筛选能够同时降解单宁和植酸的微生物,本实验利用富集培养技术,分离、筛选、鉴定土壤中的单宁和植酸降解菌,并研究其在液态发酵下的产酶能力。结果显示,从土壤中共获得109株纯菌落,包括39株细菌、46株酵母菌以及24株霉菌。分别用单宁筛选性培养基和植酸筛选性培养基筛选上述菌株,获得27株植酸降解菌和14株单宁降解菌,其中霉菌M-6、M-3和M-1可以同时分解单宁和植酸,且霉菌M-6的水解圈直径大于M-3和M-1。在液态发酵条件下,随着发酵温度的升高(20~35°C),霉菌M-6产单宁酶和植酸酶的活力呈现先升高而后降低的趋势,在发酵温度为30°C时达到最高值(P0.05)。随着发酵p H的升高(p H 4~7),霉菌M-6产单宁酶和植酸酶的活力呈先升高后降低的趋势(P0.05);其中单宁酶活力在发酵p H值为5时达到最高值,显著高于其他处理组(P0.05);而植酸酶活力在发酵p H值为5时达到最高值,显著高于发酵p H 4和7处理组(P0.05),但与发酵p H 6处理组差异不显著(P0.05)。通过菌落和菌株形态学以及分子测序方法,鉴定M-6为黑曲霉。因此,本研究从土壤中分离筛选出3株(M-6、M-3和M-1)能够同时水解单宁和植酸的降解菌,在液态发酵条件下,黑曲霉M-6产单宁酶和植酸酶的最佳发酵温度为30°C,最佳发酵p H值为5。
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| 关 键 词： | 降解微生物 水产饲料 单宁 植酸 液态发酵 |
| 收稿时间： | 2015/5/10 0:00:00 |
| 修稿时间： | 2016/6/20 0:00:00 |
Screening and identification of tannic acid and phytic acid degradative microorganisms from soil and the submerged fermentation study |
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| WANG Xiaodong,HU Haibin,MAI Kangsen,ZHANG Yanjiao,XU Wei,AI Qinghui and ZHANG Wenbing.Screening and identification of tannic acid and phytic acid degradative microorganisms from soil and the submerged fermentation study[J].Journal of Fisheries of China,2016,40(10):1634-1642. |
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| Authors: | WANG Xiaodong HU Haibin MAI Kangsen ZHANG Yanjiao XU Wei AI Qinghui and ZHANG Wenbing |
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| Institution: | Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China,Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China,Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China,Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China,Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China,Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China and Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, and the Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China |
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| Abstract: | |
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| Keywords: | degradative microorganism aqua-feed tannic acid phytic acid submerged fermentation |
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