嗜麦芽假单胞菌脂多糖的制备及其在卵形鲳鲹中的免疫效应

从海南养殖卵形鲳致病嗜麦芽假单胞菌细胞壁中提取脂多糖 ,分别用一次腹腔注射免疫法、加强腹腔注射免疫法和口服法对健康卵形鲳进行免疫接种 ,结果表明 ,以这 3种方法接种脂多糖 2～ 12周 ,实验鱼类对嗜麦芽假单胞菌的血清凝集抗体效价及其对嗜麦芽假单胞菌病的抵抗力均有显著提高。此外 ,卵形鲳在接种嗜麦芽假单胞菌脂多糖后 ,其血液中白细胞的吞噬活性也有明显增加

Preparation of Pseudomonas maltophilia lipopolysaccharidae and immunological analysis of the lipopolysaccharide against Trachinotus ovatus

The lipopolysaccharide (LPS) was isolated from the cell wall of a bacterial pathogen Pseudomonas maltophilia which was separated from Trachinotus ovatus cultured in Hainan Province. And then, it was immunized to healthy Trachinotus ovatus by the single i.p. injection, double i.p. injection and oral administration, respectively. The results showed that, within 2-12 weeks after the fish was inoculated with LPS, its agglutinating antibody titers against dead Pseudomonas maltophilia and its resistance to the bacterial pathogen Pseudomonas maltophilia increased significantly. Moreover, the phagocytic activity of the leucocytes of Trachinotus ovatus also increased after it was immunized with LPS prepared from Pseudomonas maltophilia.