| 泥鳅胚胎玻璃化液超低温冷冻保存研究 |
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| 引用本文： | 章龙珍.泥鳅胚胎玻璃化液超低温冷冻保存研究[J].水产学报,2002,26(3):213-218. |
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| 作者姓名： | 章龙珍 |
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| 作者单位： | 1. 中国水产科学研究院长江水产研究所,湖北荆州,434000;中国水产科学研究院东海水产研究所,上海,200090
2. 中国水产科学研究院长江水产研究所,湖北荆州,434000 |
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| 基金项目： | 国家“九五”攻关项目子专题 ( 96 -0 0 8-0 1-0 3-0 3) |
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| 摘 要： | 泥鳅胚胎（胚孔封闭期）用10＃玻璃化液作为低温保护剂，经低温（－196℃）冷冻保存17h后，38℃水浴解冻，解冻后的胚胎获得了成活。在稀释液B1、B2、B3中分别有26．7％，15．0％，3．0％成活的胚胎。解冻后的胚孔封闭期胚胎经过50h的培养，胚胎从胚孔封闭期发育至尾鳍出现期。
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| 关 键 词： | 泥鳅胚胎 玻璃化液 超低温冷冻保存 |
| 文章编号： | 1000-0615(2002)03-0213-06 |
| 收稿时间： | 4/9/2014 12:00:00 AM |
| 修稿时间： | 2001年8月24日 |
Cryopreservation of loach, Misgurnus anguillicaudatus,embryos by vitrification |
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| ZHANG Long-zhen.Cryopreservation of loach, Misgurnus anguillicaudatus,embryos by vitrification[J].Journal of Fisheries of China,2002,26(3):213-218. |
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| Authors: | ZHANG Long-zhen |
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| Institution: | ZHANG Long-zhen 1,2,LU Da-chun 1,LIU Ling 1,GUO Feng 1,ZHANG Jie-ming 1 |
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| Abstract: | Use of a vitrified solut ion ( composition 10) as a cryoprotectant for the cryopreservation of loach
embryos is studied in this paper. Using this vitrif ied solution, embryos were preserved at a temperature of
- 196 e for 17 hours. Afterward, the preserved embryos were thawed using thawing so lution in warm water
( 38 e ) , and revival embryos were successfully obtained. During thawing, we compared 3 kinds of thawing
solution compositions ( B1, B2 and B3) , and the survival rates of the preserved embryos after thawing in B1,
B2 and B3 thawing solutions were 26. 7% , 15. 0% and 3. 0% respectively. After thaw ing, it took 50 hours for
the development of revival embryos from the interval of blastopore closure to the interval of caudal fin
appearance. |
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| Keywords: | loach embryos cryopreservation cryoprotectant vitrified solution |
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