| 半滑舌鳎三倍体鱼苗的人工诱导与鉴定 |
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| 引用本文： | 陈松林,李文龙,季相山,谢明树,徐营,邓寒.半滑舌鳎三倍体鱼苗的人工诱导与鉴定[J].水产学报,2011,35(6):925-931. |
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| 作者姓名： | 陈松林 李文龙 季相山 谢明树 徐营 邓寒 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛,266071
2. 中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛,266071;上海海洋大学水产与生命学院,上海,201306 |
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| 基金项目： | 公益性行业(农业)科研专项经费资助(200903046) |
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| 摘 要： | 针对半滑舌鳎雌雄个体生长差异过大、雌性性腺发育成熟后腹部凸起影响舌鳎生长和商品鱼质量等问题,开展了人工诱导半滑舌鳎三倍体的研究。采用静水压处理抑制半滑舌鳎受精卵第二极体排出进行染色体加倍,筛选出有效的静水压处理强度及其持续时间。结果表明,孵化水温23 ℃左右时,授精后5 min,采用36 MPa的静水压压力,休克处理4 min,三倍体诱导率最高,达到100%。采用该诱导条件,大批量获得了半滑舌鳎三倍体鱼苗。采用流式细胞仪分析了三倍体鱼苗细胞DNA含量,表明三倍体鱼苗细胞DNA含量为二倍体对照鱼苗的1.5倍。通过染色体分析表明,三倍体鱼苗的染色体数为63条,而二倍体鱼苗的染色体数为42条。
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| 关 键 词： | 半滑舌鳎 三倍体 静水压处理 |
| 收稿时间： | 1/15/2011 5:59:41 PM |
| 修稿时间： | 4/6/2011 12:04:00 PM |
Induction and identification of artificial triploid fry in Cynoglossus semilaevis |
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| CHEN Song-lin,LI Wen-long,JI Xiang-shan,XIE Ming-shu,XU Ying and DENG Han.Induction and identification of artificial triploid fry in Cynoglossus semilaevis[J].Journal of Fisheries of China,2011,35(6):925-931. |
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| Authors: | CHEN Song-lin LI Wen-long JI Xiang-shan XIE Ming-shu XU Ying and DENG Han |
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| Institution: | Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences |
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| Abstract: | |
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| Keywords: | Cynoglossus semilaevis all-triploids pressure shock |
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