| CYP3A65及上游核受体基因PXR和AHR2在斑马鱼生长发育阶段的表达 |
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| 引用本文： | 祁美娟,石美智,张婧娴,陈君君,张湘奇,杨姣,郭澄,韩永龙.CYP3A65及上游核受体基因PXR和AHR2在斑马鱼生长发育阶段的表达[J].水产学报,2019,43(6):1416-1422. |
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| 作者姓名： | 祁美娟 石美智 张婧娴 陈君君 张湘奇 杨姣 郭澄 韩永龙 |
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| 作者单位： | 上海海洋大学食品学院, 上海 201306,上海健康医学院附属第六人民医院东院, 药剂科, 上海 201306;上海交通大学附属第六人民医院, 药剂科, 上海 200233,上海交通大学附属第六人民医院, 药剂科, 上海 200233,上海健康医学院附属第六人民医院东院, 药剂科, 上海 201306;上海交通大学附属第六人民医院, 药剂科, 上海 200233,上海交通大学附属第六人民医院, 药剂科, 上海 200233,上海健康医学院附属第六人民医院东院, 药剂科, 上海 201306;上海交通大学附属第六人民医院, 药剂科, 上海 200233,上海交通大学附属第六人民医院, 药剂科, 上海 200233,上海健康医学院附属第六人民医院东院, 药剂科, 上海 201306;上海交通大学附属第六人民医院, 药剂科, 上海 200233 |
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| 基金项目： | 国家自然科学基金（81773949）；浦东科经委（PKJ2017-Y07）；浦东卫计委（PW2017E-2）；上海市第六人民医院东院院人才（2016018） |
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| 摘 要： | 为了选择处于最佳生长阶段的斑马鱼用于药物代谢研究,本实验考察不同生长阶段斑马鱼全鱼、肝脏和肠道组织中CYP3A65及其上游核受体基因(PXR、AHR2) mRNA表达水平的变化规律。实验测定CYP3A65、PXR和AHR2相对表达量,并通过比较核受体基因与代谢酶基因mRNA之间的相关性来证实核受体基因的调控作用。结果显示,144 hpf和75 dpf两个生长阶段的斑马鱼中CYP3A65、PXR和AHR2 mRNA表达量最高;斑马鱼生长阶段中代谢酶基因和核受体基因的表达趋势一致,且受肝脏、肠道组织占全鱼比重的影响。研究表明,幼鱼期144 hpf斑马鱼及稚鱼期75 dpf的斑马鱼最适用于CYP3A65代谢研究;CYP3A65与PXR、CYP3A65与AHR2的mRNA表达之间有显著的相关性,与AHR2的相关性更显著。
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| 关 键 词： | 斑马鱼 CYP3A65 PXR AHR2 mRNA表达 发育阶段 |
| 收稿时间： | 2018/9/28 0:00:00 |
| 修稿时间： | 2018/12/3 0:00:00 |
Expression of metabolic enzyme CYP3A65 and its upstream nuclear receptor PXR and AHR2 genes during zebrafish (Danio rerio) developmental stages |
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| QI Meijuan,SHI Meizhi,ZHANG Jingxian,CHEN Junjun,ZHANG Xiangqi,YANG Jiao,GUO Cheng and HAN Yonglong.Expression of metabolic enzyme CYP3A65 and its upstream nuclear receptor PXR and AHR2 genes during zebrafish (Danio rerio) developmental stages[J].Journal of Fisheries of China,2019,43(6):1416-1422. |
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| Authors: | QI Meijuan SHI Meizhi ZHANG Jingxian CHEN Junjun ZHANG Xiangqi YANG Jiao GUO Cheng and HAN Yonglong |
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| Institution: | College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China,Department of Pharmacy, Shanghai University ofMedicine & Health Sciences Affiliated Sixth People''s Hospital East Campus, Shanghai 201306, China;Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China,Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China,Department of Pharmacy, Shanghai University ofMedicine & Health Sciences Affiliated Sixth People''s Hospital East Campus, Shanghai 201306, China;Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China,Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China,Department of Pharmacy, Shanghai University ofMedicine & Health Sciences Affiliated Sixth People''s Hospital East Campus, Shanghai 201306, China;Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China,Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China and Department of Pharmacy, Shanghai University ofMedicine & Health Sciences Affiliated Sixth People''s Hospital East Campus, Shanghai 201306, China;Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai 200233, China |
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| Abstract: | |
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| Keywords: | Danio rerio CYP3A65 PXR AHR2 mRNA expression developmental stages |
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