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n-3/n-6 HUFA对许氏平鲉幼鱼生长、体组成及组织脂肪酸组成的影响
引用本文:马长兴,王际英,李宝山,王成强,沈钰博,刘财礼,周莹.n-3/n-6 HUFA对许氏平鲉幼鱼生长、体组成及组织脂肪酸组成的影响[J].水产学报,2019,43(10):2138-2153.
作者姓名:马长兴  王际英  李宝山  王成强  沈钰博  刘财礼  周莹
作者单位:上海海洋大学水产与生命学院, 水产科学国家级实验教学示范中心, 农业农村部鱼类营养与环境生态研究中心, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006,山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006,山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006,山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006,上海海洋大学水产与生命学院, 水产科学国家级实验教学示范中心, 农业农村部鱼类营养与环境生态研究中心, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006,上海海洋大学水产与生命学院, 水产科学国家级实验教学示范中心, 农业农村部鱼类营养与环境生态研究中心, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006,上海海洋大学水产与生命学院, 水产科学国家级实验教学示范中心, 农业农村部鱼类营养与环境生态研究中心, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006
基金项目:烟台市科技计划(2017ZH068)
摘    要:为研究饲料n-3/n-6高不饱和脂肪酸(HUFA)对许氏平鲉幼鱼生长、体组成及组织脂肪酸组成的影响,配制了6种n-3/n-6 HUFA(D1:14.28,D2:9.26,D3:5.66,D4:3.06,D5:2.02,D6:1.50)的等氮等脂的实验饲料。以许氏平鲉幼鱼(36.30±0.03) g为研究对象,在网箱中养殖65 d,分为6实验组,每组设3个重复,每个重复30尾鱼。结果发现:①饲料n-3/n-6 HUFA对许氏平鲉幼鱼的成活率无显著影响。随着n-3/n-6 HUFA降低,幼鱼增重率呈先上升后下降趋势,饲料系数呈相反趋势,D2和D3组的增重率显著高于各组。②全鱼和肌肉粗脂肪含量呈先上升后下降趋势,分别在D2、D3组达到最大值。肝脏粗脂肪含量呈先下降后上升趋势,D2组显著小于其他各组。③各组织C20:4n-6含量随n-3/n-6 HUFA的降低均呈上升趋势,而C20:5n-3、C22:6n-3和n-3/n-6 HUFA整体呈下降趋势。④鱼体脂肪酸组成受饲料影响程度由大到小依次为腹脂、肌肉、全鱼、肝脏,且各组织C20:5n-3与饲料C20:4n-6均呈显著负相关。研究表明,在本实验条件下,饲料中适宜比例(5.66~9.26)的n-3/n-6 HUFA显著提高实验鱼的生长,改变体组成及组织脂肪酸组成,以增重率和饲料系数作评价指标,经一元二次回归分析得许氏平鲉幼鱼饲料中n-3/n-6 HUFA的适宜比例分别是8.93和8.70。

关 键 词:许氏平鲉  n-3/n-6  HUFA  生长  体组成  脂肪酸
收稿时间:2019/9/19 0:00:00
修稿时间:2019/9/29 0:00:00

Effects of dietary n-3/n-6 HUFA on growth, body composition and fatty acid composition of tissue in juvenile rockfish (Sebastes schlegeli)
MA Changxing,WANG Jiying,LI Baoshan,WANG Chengqiang,SHEN Yubo,LIU Caili and ZHOU Ying.Effects of dietary n-3/n-6 HUFA on growth, body composition and fatty acid composition of tissue in juvenile rockfish (Sebastes schlegeli)[J].Journal of Fisheries of China,2019,43(10):2138-2153.
Authors:MA Changxing  WANG Jiying  LI Baoshan  WANG Chengqiang  SHEN Yubo  LIU Caili and ZHOU Ying
Institution:National Demonstration Center for Experimental Fisheries Science Education, Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breedin, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China,Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China,Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China,Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China,National Demonstration Center for Experimental Fisheries Science Education, Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breedin, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China,National Demonstration Center for Experimental Fisheries Science Education, Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breedin, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China and National Demonstration Center for Experimental Fisheries Science Education, Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breedin, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute, Yantai 264006, China
Abstract:
Keywords:Sebastes schlegeli  n-3/n-6 HUFA  growth  body composition  fatty acid
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