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| 黄颡鱼遗传图谱构建及生长相关性状的QTL定位 | |
| 引用本文： | 葛学亮,尹洪滨,毕冰,孙中武.黄颡鱼遗传图谱构建及生长相关性状的QTL定位[J].水产学报,2010,34(2):185-193. |
| 作者姓名： | 葛学亮 尹洪滨 毕冰 孙中武 |
| 作者单位： | 1. 东北林业大学生命科学学院,黑龙江,哈尔滨150040;中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨150070 2. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨150070 3. 东北林业大学生命科学学院,黑龙江,哈尔滨150040 |
| 基金项目： | 黑龙江省科技厅资助项目(GZ03B109); 国家技术基础条件平台建设项目(2006DKA30470-005) |
| 摘 要： | 以野生(♂)和人工养殖(♀)黄颡鱼杂交的100个F1个体为作图群体,用SSR、SRAP和TRAP3种DNA分子标记技术构建黄颡鱼的遗传连锁图谱。图谱整合了13个SSR标记,89个SRAP标记,26个TRAP标记。其中雌性框架图谱包括16个连锁群,图谱的长度为585.5cM;雄性框架图谱包括15个连锁群,图谱的长度为752.3cM;共享框架图谱包括5个连锁群,图谱的长度为231.3cM。用该连锁图谱对黄颡鱼的5个生长相关性状进行QTL扫描,在雌性图谱上检测到1个头宽的QTL,定位于第七连锁群上,LOD值为3.2,可解释的表型变异为13%。在雄性图谱上分别检测到1个体高和体长的QTL,均定位于第一连锁群上。体高QTL的LOD值为2.4,可解释的表型变异为12%。全长QTL的LOD值为2.1,可解释的表型变异为11%。3个QTL均可用于黄颡鱼的生长性状的标记辅助育种。 |
| 关 键 词： | 黄颡鱼 遗传图谱 简单重复序列 序列相关扩增多态性 目标区域扩增多态性 数量性状遗传位点 |
| 收稿时间： | 8/5/2009 12:00:00 AM |
| 修稿时间： | 2009/11/9 0:00:00 |
A preliminary research of genetic linkage map construction and QTL analysis for growth related traits in yellow catfish (Pelteobagrus fulvidraco) |
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| GE Xueliang,YIN Hongbin,BI Bing and SUN Zhongwu.A preliminary research of genetic linkage map construction and QTL analysis for growth related traits in yellow catfish (Pelteobagrus fulvidraco)[J].Journal of Fisheries of China,2010,34(2):185-193. | |
| Authors: | GE Xueliang YIN Hongbin BI Bing and SUN Zhongwu |
| Institution: | GE Xue-liang1,2,YIN Hong-bin2,BI Bing1,SUN Zhong-wu1 (1.College of Life Science,Northeast Forestry University,Harbin 150040,China,(2.Heilongjiang River Fishery Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China) |
| Abstract: | Genetic linkage maps of yellow catfish (Pelteobagrus fulvidraco) were constructed using SSR,SRAP and TRAP markers and a pseudo-testcross mapping strategy. A segregating population including 100 progenies was sampled within the first filial generation obtained by artificial fertilization between a male parent and a female parent from wild and breeding populations respectively. Totally,13 SSR,89 SRAP and 26 TRAP markers were used to construct a genetic map. Female map included 16 linkage groups,covering a tot... |
| Keywords: | Pelteobagrus fulvidraco genetic linkage map SSR SRAP TRAP QTL |
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