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亚硝酸盐氮胁迫对鳙血液生化指标以及组织HSP70 mRNA表达水平的影响
引用本文:林艳,缪凌鸿,盘文静,戈贤平,任鸣春,刘波,梁化亮,赵振新.亚硝酸盐氮胁迫对鳙血液生化指标以及组织HSP70 mRNA表达水平的影响[J].水产学报,2017,41(8):1192-1202.
作者姓名:林艳  缪凌鸿  盘文静  戈贤平  任鸣春  刘波  梁化亮  赵振新
作者单位:南京农业大学渔业学院, 江苏 无锡 214081,南京农业大学渔业学院, 江苏 无锡 214081;中国水产科学研究院淡水渔业研究中心, 农业部淡水鱼类遗传育种和养殖生物学重点开放实验室, 江苏 无锡 214081,南京农业大学渔业学院, 江苏 无锡 214081,南京农业大学渔业学院, 江苏 无锡 214081;中国水产科学研究院淡水渔业研究中心, 农业部淡水鱼类遗传育种和养殖生物学重点开放实验室, 江苏 无锡 214081,中国水产科学研究院淡水渔业研究中心, 农业部淡水鱼类遗传育种和养殖生物学重点开放实验室, 江苏 无锡 214081,南京农业大学渔业学院, 江苏 无锡 214081;中国水产科学研究院淡水渔业研究中心, 农业部淡水鱼类遗传育种和养殖生物学重点开放实验室, 江苏 无锡 214081,南京农业大学渔业学院, 江苏 无锡 214081,南京农业大学渔业学院, 江苏 无锡 214081
基金项目:现代农业产业技术体系大宗淡水鱼专项(CARS-46);中央级公益性科研院所基本科研业务费专项(2015GBFM25)
摘    要:为了探讨亚硝酸盐氮胁迫对鳙初均重:(180.05±0.092)g]血液生化指标和组织热休克蛋白70基因表达水平的影响,实验选用170尾鳙,暴露于48.634 mg/L亚硝酸盐氮96 h后,进行96 h恢复实验。并在胁迫0、6、12、24、48、72和96 h以及恢复12、24、48、72和96 h时采集样品。结果显示,胁迫6 h后,血清皮质醇含量和谷丙转氨酶活性显著升高;胁迫12 h后,血清中葡萄糖含量显著升高;胁迫24 h后,血清总胆固醇含量开始显著降低;胁迫48 h后,血清总蛋白和甘油三酯含量开始显著降低,三碘甲状腺原氨酸含量和谷草转氨酶活性则显著升高;胁迫72 h后,血清低密度脂蛋白和高密度脂蛋白含量开始显著降低。恢复96 h后,鱼体血清生理指标大多都已恢复至胁迫前水平,而组织热休克蛋白70 mRNA表达水平的差异显示,头肾抗亚硝酸盐氮响应最快,其次为鳃和肠道,而脾脏热休克蛋白70 mRNA表达水平在恢复12 h时显著升高;恢复96 h后,除鳃组织外,肾脏、肠道和脾脏的热休克蛋白70 mRNA表达量均恢复至胁迫前水平。研究表明,亚硝酸盐氮对鳙的蛋白质代谢、脂质代谢和糖代谢均产生影响,并且诱导部分组织热休克蛋白70 mRNA的表达,而鳙对水体中高浓度的亚硝酸盐氮应激6 h即可产生快速应答,并启动防损伤自我保护机制,促进新陈代谢水平,保护机体免受应激损伤。

关 键 词:  亚硝酸盐氮  胁迫  血液生化指标  应激激素  HSP70
收稿时间:2016/3/10 0:00:00
修稿时间:2016/10/10 0:00:00

Effects of nitrite nitrogen stress on blood biochemical indictors and HSP70 mRNA expression level of Aristichthys nobilis
LIN Yan,MIAO Linghong,PAN Wenjing,GE Xianping,REN Mingchun,LIU Bo,LIANG Hualiang and ZHAO Zhenxin.Effects of nitrite nitrogen stress on blood biochemical indictors and HSP70 mRNA expression level of Aristichthys nobilis[J].Journal of Fisheries of China,2017,41(8):1192-1202.
Authors:LIN Yan  MIAO Linghong  PAN Wenjing  GE Xianping  REN Mingchun  LIU Bo  LIANG Hualiang and ZHAO Zhenxin
Institution:Fishery College, Nanjing Agriculture University, Wuxi 214081, China,Fishery College, Nanjing Agriculture University, Wuxi 214081, China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China,Fishery College, Nanjing Agriculture University, Wuxi 214081, China,Fishery College, Nanjing Agriculture University, Wuxi 214081, China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China,Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China,Fishery College, Nanjing Agriculture University, Wuxi 214081, China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China,Fishery College, Nanjing Agriculture University, Wuxi 214081, China and Fishery College, Nanjing Agriculture University, Wuxi 214081, China
Abstract:In order to study the effects of nitrite nitrogen stress on blood biochemical indictors and HSP70 mRNA expression level of Aristichthys nobilis initial average weight (180.05±0.092) g], 170 individuals of A. nobilis were exposed to water body with nitrite nitrogen concentration of 48.634 mg/L for 96 hours, and then a 96-hour recovery experiment was carried out. Blood and tissues of gill, head kidney, intestines and spleen were sampled at 0, 6, 12, 24, 48, 72, 96 h after stress, and at 12, 24, 48, 72, 96 h after recovery. For the stress and recovery experiment process, the results showed serum cortisol and ALT activity significantly increased first, after being stressed for 6 h. Thereafter, serum GLU presented significant increase at 12 h. Whereafter, serum CHOL, TP and TG decreased significantly and T3, AST increased significantly, while serum LDL and HDL decreased significantly at 72 h. For the recovery experiment, A. nobilis were recovered from nitrite nitrogen stress after 96 h. The HSP70 mRNA expression of different tissues showed that head kidney presented the fastest response to nitrite nitrogen stress, then gill, intestine and spleen. For recovering 96 h, HSP70 mRNA expression of head kidney, intestine and spleen returned to normal. The result suggested that nitrite nitrogen affected the protein metabolism, lipid metabolism and glycometabolism of A. nobilis, and also influenced the HSP70 mRNA expression of different tissues. A. nobilis showed rapid response to stress of high concentrations of water nitrite nitrogen after 6 h, so as to regulate physiological metabolism, and improve protection from stress injury and damage.
Keywords:Aristichthys nobilis  nitrite nitrogen  stress  blood biochemical indictors  stress hormone  HSP70
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